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首页> 外文期刊>African Journal of Biotechnology >Optimization of extraction parameters for trehalose from beer waste brewing yeast treated by high-intensity pulsed electric fields (PEF)
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Optimization of extraction parameters for trehalose from beer waste brewing yeast treated by high-intensity pulsed electric fields (PEF)

机译：高强度脉冲电场（PEF）处理啤酒废料酿造酵母中海藻糖的提取参数优化

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High-intensity pulsed electric fields (PEF) was applied to treat beer waste brewing yeasts (BWBY) to improve the permeability of yeast cell membrane in order to extract trehalose. Several independent variables such as pH (3 to 7), electric field strength (0 to 50 kV/cm), pulse numbers (0 to 10) and liquid-solid ratio (20: 1 to 60: 1) were investigated. The optimal parameters were obtained by one-factor-at-a-time (OFAT) experiment and quadratic regression orthogonal design. The results showed that under the conditions of electric fields intensity of 19.97 kV/cm, pulse number of 6, and liquid-solid ratio of 30:1, the extraction rate of trehalose could reach 2.635%. In addition, the extraction efficiency of trehalose treated by PEF was compared with other two trehalose extraction methods, example?microwave and ultrasound, and the efficiency of PEF was found to be 103.15 μg/s; it was 15.96 times higher than microwave and 34.08 times higher than ultrasound. This demonstrated that the PEF could be regarded as a promising technique for bio-material extraction.
机译：为了提取海藻糖，采用高强度脉冲电场（PEF）处理啤酒废料酿造酵母（BWBY），以提高酵母细胞膜的通透性。研究了几个独立变量，例如pH（3至7），电场强度（0至50 kV / cm），脉冲数（0至10）和液固比（20：1至60：1）。最佳参数是通过一次一因素（OFAT）实验和二次回归正交设计获得的。结果表明，在电场强度为19.97 kV / cm，脉冲数为6，液固比为30：1的条件下，海藻糖的提取率为2.635％。另外，将PEF处理的海藻糖的提取效率与微波，超声等两种海藻糖提取方法进行了比较，发现PEF的效率为103.15μg/ s。比微波高15.96倍，比超声高34.08倍。这表明PEF可以被认为是生物材料提取的有前途的技术。

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