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首页> 外文期刊>African Journal of Biotechnology >Production of single cell protein (SCP) and essentials amino acids from Candida utilis FMJ12 by solid state fermentation using mango waste supplemented with nitrogen sources
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Production of single cell protein (SCP) and essentials amino acids from Candida utilis FMJ12 by solid state fermentation using mango waste supplemented with nitrogen sources

机译:利用芒果废料和氮源进行固态发酵,从util假丝酵母FMJ12生产单细胞蛋白(SCP)和必需氨基酸

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In Burkina Faso, deficiency of amino acids in protein is becoming a major healthy public problem. This study was purposed to optimize essentials amino acids in single cell protein (SCP) by supplementing different nitrogen sources during fermentation of mango waste with Candida utilis FJM12. Analytical methods were used to determine biomass yield, chemical composition and amino acids profile of SCP. The principal component analysis (PCA) method was performed to identify the nitrogen source which exhibited best rate of SCP. The maximum biomass yield (6.48±0.03 g/L) exhibited 9.65±0.36% (w/w) of ash, while using yeast extract. The proximate composition of SCP revealed 56.40±1.30, 13.25±0.11, 3.80±0.10, and 6.60±0.25% (w/w), respectively for crude protein, lipids, carbohydrates, and nucleic acid content. PCA showed a strong correlation between yeast extract and Ammonium sulphate and demonstrated their positive influence to increase the rate of SCP and essentials amino acids as compared to Food and Agriculture Organization (FAO) recommendation. These results demonstrated that C. utilis FJM12 could be suitable for essentials amino acids.
机译:在布基纳法索,蛋白质中氨基酸的缺乏正成为一个主要的健康公共问题。这项研究旨在通过在利用假丝酵母FJM12发酵芒果废料过程中补充不同的氮源来优化单细胞蛋白质(SCP)中的必需氨基酸。使用分析方法确定SCP的生物量产量,化学组成和氨基酸谱。进行了主成分分析(PCA)方法以鉴定表现出最佳SCP发生率的氮源。使用酵母提取物时,最大生物量产量(6.48±0.03 g / L)表现出9.65±0.36%(w / w)的灰分。 SCP的最接近组成分别为粗蛋白,脂质,碳水化合物和核酸含量,分别为56.40±1.30、13.25±0.11、3.80±0.10和6.60±0.25%(w / w)。 PCA显示出酵母提取物和硫酸铵之间有很强的相关性,与食品和农业组织(FAO)的建议相比,它们显示出对增加SCP和必需氨基酸率的积极影响。这些结果表明,C.utilis FJM12可能适合必需氨基酸。

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