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首页> 外文期刊>African Journal of Biotechnology >Multiplex polymerase chain reaction for detection and characterization of shiga toxigenic Escherichia coli (STEC)
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Multiplex polymerase chain reaction for detection and characterization of shiga toxigenic Escherichia coli (STEC)

机译：多重聚合酶链反应用于检测和鉴定志贺毒素性大肠杆菌（STEC）

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Escherichia coli?is ubiquitous in the cow's environment that is contaminated by feces, and it is also a frequent cause of bovine mastitis. Thus, the present study was targeted at the rapid detection and characterization of shiga toxigenic?E. coli(STEC) in bovine fecal and milk samples. Twenty two strains of?E. coli?(39.29%) were isolated from 56 diarrheic calves, while only 5 strains (20.83%) were isolated from apparently normal contact calves. Moreover, 20 strains of?E. coli?(25%) were isolated from milk samples collected from 80 animals suffering from mastitis and subclinical mastitis.?E. coli?serovars yielded from bacteriologi-cal examination of milk samples were similar to that of fecal samples. Serogroup-specific multiplex polymerase chain reaction (PCR) assay could detect all the bacteriologically positive samples as well as 4 strains (7.98%), O157:H7 and 3 strains (5.36%), O111 from diarrheic calves and 2 strains (8.33%), O111 from normal calves. Such samples were proved to be negative by bacteriological examination. Multiplex PCR for detection of genes encoding accessory STEC virulence factors, such as shiga toxin type-2 (stx2)?and intimin gene (eaeA) revealed the specificity of such gene to O157:H7 serovars and small number of other sero-groups.
机译：大肠杆菌在被粪便污染的奶牛环境中无处不在，也是牛乳腺炎的常见病因。因此，本研究的目标是快速检测和鉴定志贺毒素。牛粪和牛奶样品中的大肠杆菌（STEC）。 22株？E。从56只腹泻小牛中分离出大肠杆菌（39.29％），而从显然正常的接触小牛中仅分离出5株（20.83％）。此外，还有20株ΔE。从80例患有乳腺炎和亚临床乳腺炎的动物的牛奶样品中分离出大肠杆菌（25％）。牛奶样品的细菌学检查产生的大肠杆菌血清型与粪便样品相似。血清群特异性多重聚合酶链反应（PCR）检测可以检测所有细菌阳性样品以及腹泻小牛的4株（7.98％），O157：H7和3株（5.36％），O111和2株（8.33％） ，O111来自普通犊牛。通过细菌学检查证明这些样品是阴性的。多重PCR检测编码辅助STEC毒力因子的基因，例如志贺毒素2型（stx2）和内膜素基因（eaeA），揭示了该基因对O157：H7血清型和少量其他血清群的特异性。

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