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首页> 外文期刊>African Journal of Biotechnology >The ultrasonic effect on the mechanism of cholesterol oxidase production by Brevibacterium sp.
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The ultrasonic effect on the mechanism of cholesterol oxidase production by Brevibacterium sp.

机译：超声对短杆菌属产生胆固醇氧化酶机制的影响。

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摘要

The effects of ultrasonic radiation on?cholesterol oxidase production byBrevibacterium?sp.?are studied in this paper. An ultrasonic wave with low intensity at 20 kHz, 200 W/ cm2?was employed to study the effects of irradiation at different lengths of time on the growth of?Brevibacterium?sp.?cells. The result showed that the production of?cholesterol oxidase?from?Brevibacterium sp.?obviously increased, and was about 18.3%, when the culture was radiated for 1 min every 4 h. In order to investigate the action of ultrasonic on cell membrane and to facilitate enzyme excretion, transmission electron microscopy (TEM) was employed.?Scanning of the bacterial cells by?TEM?also first revealed that holes appeared in the cytoplasmic region, then excluded from the cell barrage, and finally?self-destructed?with higher irradiating dosage.
机译：本文研究了超声波对短杆菌属细菌产生胆固醇氧化酶的影响。用20 kHz，200 W / cm2？的低强度超声波研究不同时间长度的辐照对短杆菌属？sp。细胞生长的影响。结果表明，每4小时辐射1分钟，短杆菌属（Brevibacterium sp。）产生的胆固醇氧化酶明显增加，约为18.3％。为了研究超声波对细胞膜的作用并促进酶的排泄，采用了透射电子显微镜（TEM）。“通过TEM扫描细菌细胞”还首先显示出在细胞质区域出现了孔，然后从孔中排除了孔。细胞弹幕，并最终以更高的辐射剂量自毁。

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