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首页> 外文期刊>African Journal of Biotechnology >Genetic diversity of male fig (Ficus carica caprificus L.) genotypes with random amplified polymorphic DNA (RAPD) markers
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Genetic diversity of male fig (Ficus carica caprificus L.) genotypes with random amplified polymorphic DNA (RAPD) markers

机译：具有随机扩增多态性DNA（RAPD）标记的雄性无花果（Ficus carica caprificus L.）基因型的遗传多样性

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摘要

The objective of this research is?to characterize and relate 43 different male fig (Ficus carica caprificus?L.) genotypes grown in Ayd?n Province, Turkey using random amplified polymorphic DNA (RAPD) markers. A total of 85 10-mer primers were tested with the RAPD technique. A total of 76 polymorphic bands out of 272 bands (27.9%) were observed from 36 primers. The molecular size varied from 200 - 3000 bp. Genotypes were placed in nine different groups in the dendrogram drawn from the similarity matrix. The closest genotypes are ‘Yanako1’ and ‘Yanako2’. The relationship between the genotypes and their respective regions supported by the dendrogram is incongruent. Polymorphic information content (PIC) values ranged from 0.16 (OPD08) to 0.50 (OPC01 and OPC04) for the polymorphic data set. The larger source of variation was observed within populations (88.58%) followed by among populations (11.42%) in the analysis of molecular variance. The relationships among genotypes were defined by the first three principle components (PC), accounting for 40.91% of the total variation at the molecular level.
机译：这项研究的目的是使用随机扩增多态性DNA（RAPD）标记来鉴定和关联在土耳其艾登省生长的43种不同的男性无花果（Ficus carica caprificus？L。）基因型。用RAPD技术测试了总共85个10-mer引物。从36个引物中观察到272条带中的76条多态性条带（占27.9％）。分子大小在200-3000 bp之间变化。从相似性矩阵得出的树状图中将基因型分为九个不同的组。最接近的基因型是“ Yanako1”和“ Yanako2”。树型图支持的基因型与它们各自区域之间的关系不一致。对于多态数据集，多态信息内容（PIC）值的范围从0.16（OPD08）到0.50（OPC01和OPC04）。在分子变异分析中，观察到较大的变异源是在群体内（88.58％），其次是群体间（11.42％）。基因型之间的关系由前三个主要成分（PC）定义，占分子水平总变异的40.91％。

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