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首页> 外文期刊>African Journal of Biotechnology >Bioconversion process of rice straw by thermotolerant cellulolytic Streptomyces viridiochromogenes under solid-state fermentation conditions for bioethanol production
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Bioconversion process of rice straw by thermotolerant cellulolytic Streptomyces viridiochromogenes under solid-state fermentation conditions for bioethanol production

机译：固态发酵条件下耐热纤维素解绿链霉菌对稻草的生物转化过程

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摘要

Enzymatic hydrolysis of the cellulose fraction of rice straw to glucose?using solid-state fermentation?for bioethanol production is a focus of current attention.?A total of 10 actinomycetes isolates were isolated from?soils and decayed rice straw.?All these isolates were purified and screened for their?cellulolytic?activity; one strain was?selected for further study and identified as?Streptomyces?viridiochromogenes.Optimization of fermentation conditions showed highest cellulolytic enzymes production on?the?5th day at pH 6.5 and at 40°C. The production of enzymes reached its maximal value at 4.0 g of rice straw/250 ml flask.?Avicelase?and total cellulase productivity were highly increased by?the?addition of NH4Cl as N-source, while?maximum activity?of CMCase was?recorded by?the?addition?of?peptone as N-source to the fermentation medium. The influence of various physico-chemical factors on enzyme activity was also investigated. The half life time of avicelase, total cellulase and CMCase at 60°C was 39.4, 50.0 and 78.58 min, respectively. A maximum of ethanol production 1.428±0.074% (v/v) by?Saccharomyces cereviseaeusing dilute acid pretreated rice straw hydrolysate with initial soluble sugar 2.340±0.072% was recorded after 2 days of fermentation.
机译：通过固态发酵将稻草的纤维素部分酶促水解为葡萄糖以生产生物乙醇是目前的关注焦点。从土壤和腐烂的稻草中分离出总共10种放线菌分离物。纯化并筛选其“纤维素分解”活性;筛选出一种菌株作进一步研究，鉴定为链霉菌型生色团。优化发酵条件显示，在pH 6.5和40°C的第5天，纤维素分解酶的产量最高。在添加稻草4.0 g / 250 ml烧瓶时，酶的产量达到最大值。通过添加NH4Cl作为氮源，Avicelase和总纤维素酶的生产率大大提高了，而CMCase的最大活性却达到了最大值。通过将pe作为氮源添加到发酵培养基中来记录。还研究了各种物理化学因素对酶活性的影响。 avicelase，总纤维素酶和CMCase在60°C的半衰期分别为39.4、50.0和78.58分钟。发酵两天后，记录了用稀酸预处理的稻草水解物与初始可溶性糖的2.340±0.072％的酿酒酵母发酵后的酿酒酵母最大乙醇产量1.428±0.074％（v / v）。

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