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首页> 外文期刊>African Journal of Biotechnology >Enhanced production and characterization of a novel -D-glucose:oxygen-1-oxidoreductase by using Aspergillus niger UV-180-C mutant strain
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Enhanced production and characterization of a novel -D-glucose:oxygen-1-oxidoreductase by using Aspergillus niger UV-180-C mutant strain

机译：黑曲霉UV-180-C突变菌株提高了新型-D-葡萄糖：-1-氧氧化还原酶的生产和表征

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Along with other?emerging health consequences, diabetes mellitus is one of the critical threats spreading all over the world with continuity. The investigation of glucose level in diabetic patients requires the key enzyme?β-D-glucose:oxygen-1-oxidoreductase/glucose oxidase, where its optimized production by mutant derived strain could affect the economic burden and accuracy of the test. Wild typeAspergillus niger?was subjected to ultraviolet radiation for enhanced production of glucose oxidase. It was found that UV-180-C?is?a potential mutant derived strain, screened by using 2-deoxy-D-glucose. Optimum production of glucose oxidase from?A. niger?UV-180-C was carried out by using CSL (2%), fermentation period (36 h), pH (5.5 and 4.5 for wild and mutant respectively), temperature (30°C), MgSO4.7H2O (0.0%), CaCO3?(0.1%), KH2PO4?(0.8 and 1.0% for wild and mutant strains), Urea (0.3%). Crude enzyme was subjected to ammonium sulfate precipitation and resulted into 145.8 UmL-1?activity. Glucose oxidase from mutant derived?A. niger?exhibited optimum pH at 6, temperature 20°C, Km?10mM and Vmax142 UmL-1. The pyridoxal phosphate caused significant inhibition to the enzyme which indicates the presence of lysyl residues near or at the active site of the enzyme from both wild and mutant derived strains.
机译：与其他新出现的健康后果一样，糖尿病也是连续性遍及全球的关键威胁之一。对糖尿病患者葡萄糖水平的研究需要关键的酶β-D-葡萄糖：氧-1-氧化还原酶/葡萄糖氧化酶，其中突变衍生菌株的优化生产可能会影响测试的经济负担和准确性。对野生型黑曲霉进行紫外线辐射以增强葡萄糖氧化酶的产生。发现通过使用2-脱氧-D-葡萄糖筛选出UV-180-Cα是一种潜在的突变体衍生菌株。从？A中最佳生产葡萄糖氧化酶。使用CSL（2％），发酵时间（36 h），pH（野生和突变体分别为5.5和4.5），温度（30°C），MgSO4.7H2O（0.0％）进行niger UV-180-C ），碳酸钙（0.1％），磷酸二氢钾（野生株和突变株分别为0.8％和1.0％），尿素（0.3％）。将粗酶进行硫酸铵沉淀，得到145.8 UmL-1？的活性。来自突变体的葡萄糖氧化酶？尼日尔在6，温度20°C，Km？10mM和Vmax142 UmL-1下显示出最佳pH值。磷酸吡ido醛对酶产生了显着的抑制作用，表明野生和突变菌株均在酶的活性位点附近或在酶的活性位点存在赖氨酰残基。

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