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首页> 外文期刊>African Journal of Biotechnology >Karyotype and nucleic acid content in Zantedeschia aethiopica Spr. and Zantedeschia elliottiana Engl.
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Karyotype and nucleic acid content in Zantedeschia aethiopica Spr. and Zantedeschia elliottiana Engl.

机译：马蹄形马齿ante中的核型和核酸含量。和马蹄莲（Zantedeschia elliottiana Engl）。

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Analysis of karyotype, nucleic deoxyribonucleic acid (DNA) content and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were performed inZantedeschia aethiopica?and?Zantedeschia elliottiana. Mitotic metaphase in both species showed 2n=32. The chromosomes of both species were quite similar with medium length ranging from 1.55 ± 0.04 to 3.85 ± 0.12 μM in?Z. aethiopica?and 2.15 ± 0.04 to 3.90 ± 0.12 μM in?Z. elliottiana. However, some differences were found in morphology and centromeric position among the chromosomes. Identification of individual chromosomes was carried out using chromosomes length, and centromeric positions. The karyotype of?Z.?aethiopica?was determined to be 2n = 32 = 14 m + 18 sm and of?Z.?elliottiana?to be 2n = 32 = 10 m + 22 sm. The 2C nuclear DNA content was found to be 3.72 ± 0.10 picograms (equivalent to 3638.16 mega base pairs) for?Z. aethiopica?and 1144.26 ± 0.05 picograms (equivalent to 1144.26 mega base pairs) for?Z. elliottiana.?Leaf protein analysis showed 11 and 9 bands for?Z. aethiopica?and?Z. elliottiana,?respectively, among which some were species specific. These results may provide useful information regarding?Zantedeschia?for the study of?taxonomic?relationships, genetics and breeding.
机译：在拟南芥和拟南芥中进行了核型，核酸脱氧核糖核酸（DNA）含量和十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）的分析。两种物种的有丝分裂中期均显示为2n = 32。两种物种的染色体都非常相似，中等长度范围为1.55±0.04至3.85±0.12μMin？Z。 aethiopica？和2.15±0.04至3.90±0.12μMin？Z。 elliottiana。但是，在染色体之间的形态和着丝粒位置中发现了一些差异。使用染色体长度和着丝粒位置来鉴定单个染色体。 ΔZ.aethiopicaΔ的核型确定为2n ＝ 32 ＝ 14m + 18sm，而Δelliottianaα的核型确定为2n ＝ 32 ＝ 10m + 22sm。对于ΔZ，发现2C核DNA含量为3.72±0.10皮克（相当于3638.16兆碱基对）。 aethiopica？和1144.26±0.05皮克（相当于1144.26兆碱基对）。 elliottiana。？叶蛋白质分析显示？Z有11和9条带。 aethiopica和Z。分别为elliottiana，其中一些是特定物种的。这些结果可能会提供有关马齿Z的有用信息，用于研究分类学关系，遗传学和育种。

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