首页> 外文期刊>African Journal of Biotechnology >Genetic diversity analysis of Nardostachys jatamansi DC, an endangered medicinal plant of Central Himalaya, using random amplified polymorphic DNA (RAPD) markers
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Genetic diversity analysis of Nardostachys jatamansi DC, an endangered medicinal plant of Central Himalaya, using random amplified polymorphic DNA (RAPD) markers

机译:喜马拉雅中部濒危药用植物Nardostachys jatamansi DC的遗传多样性分析,使用随机扩增多态DNA(RAPD)标记

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The genetic diversity analysis of eight populations of?Nardostachys jatamansi?DC.collected from different altitude of Central Himalaya has been attempted using 24 sets of?random amplified polymorphic DNA (RAPD) primers. These sets of RAPD marker generated a total of 346 discernible and reproducible bands across the analysed population with 267 polymorphic and 75 monomorphic bands. The unweighted pair group method with arithmetic average (UPGMA) cluster analysis revealed three distinct clusters: I, II and III. The cluster I was represented by?N. jatamansi?population collected from Panwali Kantha (3200 m asl) and Kedarnath (3584 m asl), India together with Jumla (2562 m asl) from Nepal. Cluster II included collections from Har Ki Doon (3400 m asl) and Tungnath (3600 m asl) from India while Cluster III was represented by collections from Munsiyari (2380 m asl), Dayara (3500 m asl) and Valley of Flowers (3400 m asl) from India. The clustering of these populations was independent of variations in altitude and geographical locations. The genetic variations observed in different populations of Jatamansi might be due to environmental influences (biotic and abiotic), rather than altitude level differences. The abiotic (geographical or climatic differentiation) and biotic (pollination between population and seed dispersal) factors might be responsible for the genetic variations among these accessions of Jatamansi.
机译:利用24套随机扩增多态性DNA(RAPD)引物,尝试对喜马拉雅中部不同海拔地区8个种群的遗传多样性进行了遗传分析。这些RAPD标记集在整个分析群体中产生了346条可分辨和可再现的条带,其中267条多态性条带和75条单态性条带。具有算术平均值(UPGMA)聚类分析的非加权对群方法揭示了三个不同的聚类:I,II和III。我代表的集群是?N。从印度的Panwali Kantha(3200 m asl)和Kedarnath(3584 m asl)以及尼泊尔的Jumla(2562 m asl)收集的jatamansi种群。第二类包括来自印度的Har Ki Doon(3400 m asl)和Tungnath(3600 m asl)的收藏,而第三类由Munsiyari(2380 m asl),Dayara(3500 m asl)和花谷(3400 m asl)来自印度。这些人口的聚集与海拔和地理位置的变化无关。在贾塔曼西不同人口中观察到的遗传变异可能是由于环境影响(生物和非生物),而不是海拔高度差异造成的。非生物(地理或气候差异)和生物(种群与种子传播之间的授粉)因素可能是贾塔曼西种质间遗传变异的原因。

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