• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Production and confirmation of clones using gynogenesis in Japanese flounder
【24h】

Production and confirmation of clones using gynogenesis in Japanese flounder

机译：利用比目鱼雌核发育的克隆的生产和确认

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Two homozygous clones of Japanese flounder were produced by the gynogenesis that suppresses the cleavage of the eggs in the mitosis and then blocks the extrusion of the second polar body in the meiosis. Microsatellite was used to identify the genetic status of maternal parents and their progenies. 21 polymorphic microsatellite markers were chosen, distributed at the distal region of the 16 linkage group. In this experiment, the two used maternal parents were completely heterozygous, while both mitotic gynogenetic diploids and their clone progenies were homozygous for each marker, demonstrating the successful development of cloning.
机译：通过雌核发育，产生了两个日本比目鱼纯合克隆，它们抑制有丝分裂中卵的分裂，然后阻止第二个极体在减数分裂中的挤出。微卫星被用于鉴定母亲父母及其后代的遗传状况。选择了21个多态性微卫星标记，分布在16个连锁组的远端区域。在该实验中，两个使用过的母体父母是完全杂合的，而有丝分裂的雌核发育二倍体及其克隆后代对于每个标记都是纯合的，证明了克隆的成功发展。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号