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首页> 外文期刊>African Journal of Biotechnology >The antimicrobial effect of Origanum compactum essential oil, nisin and their combination against Escherichia coli in tryptic soy broth (TSB) and in sheep natural sausage casings during storage at 25 and 7C
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The antimicrobial effect of Origanum compactum essential oil, nisin and their combination against Escherichia coli in tryptic soy broth (TSB) and in sheep natural sausage casings during storage at 25 and 7C

机译：牛至精油，乳链菌肽及其组合在25°C和7°C下储存期间在胰蛋白酶大豆肉汤（TSB）和绵羊天然肠衣中对大肠杆菌的抗菌作用

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摘要

The aim of this study was to investigate the antimicrobial effect of oregano essential oil (EO) at 0.015, 0.03 or 0.06%, nisin at 800 or 1600 IU/g, and their combination onEscherichia coli?in tryptic soy broth (TSB) and in sheep natural sausage casings. At 0.015%, EO presented a weak antibacterial activity against the pathogen in TSB. Consequently, only the levels of 0.03 and 0.06% were examined in casings. EO at 0.03 or 0.06% showed an inhibitory activity against?E. coli?O157:H7,?E. coli?ATCC 25922 and?E. coli?cocktail during storage at 25°C and at 7° C in casings. Treatment of casings or in TSB with nisin at 800 or 1600 IU/g did not show any antibacterial activity against?E. coli?strains. Synergy between EO and nisin?was?observed in both TSB and natural casings. The combination of EO at 0.03 or 0.06% and nisin at 800 or 1600 IU/g presented an additive effect against the pathogen, during storage at 25°C and at 7°C.
机译：这项研究的目的是研究牛至精油（EO）在0.015、0.03或0.06％，乳酸链球菌肽在800或1600 IU / g时的抗菌作用，以及它们的组合对胰蛋白酶大豆肉汤（TSB）和胰蛋白酶在大豆中的抗菌作用。绵羊天然肠衣。 EO含量为0.015％时，对TSB中的病原体表现出较弱的抗菌活性。因此，在外壳中仅检查了0.03和0.06％的水平。在0.03或0.06％的EO显示出对ΔE的抑制活性。大肠杆菌O157：H7，E。大肠杆菌ATCC 25922和E.在25°C和7°C的肠衣中储存期间的大肠杆菌鸡尾酒。用乳链菌肽以800或1600 IU / g的剂量处理肠衣或在TSB中未显示出对？E的任何抗菌活性。大肠杆菌菌株。在TSB和天然肠衣中均观察到EO和乳链菌肽之间的协同作用。在25°C和7°C下储存期间，0.03或0.06％的EO和800或1600 IU / g的乳链菌肽的组合对病原体具有累加作用。

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