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首页> 外文期刊>African Journal of Biotechnology >Growth and exopolysaccharide production by Lactobacillus fermentum F6 in skim milk
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Growth and exopolysaccharide production by Lactobacillus fermentum F6 in skim milk

机译：脱脂乳中发酵乳杆菌F6的生长和胞外多糖的产生

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摘要

Growth and exopolysaccharide (EPS) production by?Lactobacillus fermentum?F6 isolated from traditional dairy products in Inner Mongolia of China were studied when the strain was grown in 10% (w/v) reconstituted skim milk under different culture conditions. The results showed that culturing of?L. fermentum?F6 at a temperature (37°C) and pH (6.5) optimal for growth was also favorable for EPS production. Supplementation in 10% (w/v) skim milk with different carbon sources (glucose, lactose, galactose and fructose) increased EPS production; glucose being more effective than other sugars. Supplementation with whey protein concentrate (WPC) at 0.5% (w/v) resulted in about two-fold increase in EPS production. A maximum of 44.49 mg/l of EPS was produced by?L. fermentum?F6 in the skim milk medium supplemented with 2% (w/v) glucose and 0.5% (w/v) WPC at 37°C and at initial pH 6.5. Monosaccharide analysis showed that the EPS of?L. fermentum?F6 was composed of glucose and galactose in a molar ratio of 4:3. The molecular mass of the EPS was determined to be 3.54 × 106?Da. A 1% (w/v) aqueous solution of the EPS showed relatively high viscosity, indicating the potential of this EPS to be used as a viscosifying agent in food products.
机译：研究了当菌株在不同培养条件下以10％（w / v）的脱脂奶生长时，从中国内蒙古传统乳制品中分离得到的发酵乳杆菌F6的生长和胞外多糖（EPS）的产生。结果表明培养ΔL。在最适合生长的温度（37°C）和pH（6.5）下，发酵菌F6也有利于EPS的生产。补充具有不同碳源（葡萄糖，乳糖，半乳糖和果糖）的10％（w / v）脱脂牛奶可增加EPS产量;葡萄糖比其他糖更有效。补充0.5％（w / v）的乳清蛋白浓缩物（WPC）可使EPS产量增加约两倍。 ？L最多可产生44.49 mg / l的EPS。补充了2％（w / v）葡萄糖和0.5％（w / v）WPC的脱脂牛奶培养基中的发酵液F6发酵液，初始pH值为6.5。单糖分析表明，EPS为？L。发酵菌F6由葡萄糖和半乳糖组成，摩尔比为4∶3。 EPS的分子量确定为3.54×106？Da。 EPS的1％（w / v）水溶液显示出较高的粘度，表明该EPS可用作食品中的增粘剂的潜力。

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