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首页> 外文期刊>African Journal of Biotechnology >Assessment of genetic diversity in Isabgol (Plantago ovata Forsk.) using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers for developing crop improvement strategies
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Assessment of genetic diversity in Isabgol (Plantago ovata Forsk.) using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers for developing crop improvement strategies

机译：使用随机扩增多态性DNA（RAPD）和简单序列间重复序列（ISSR）标记评估伊萨博格尔州（伊斯坦堡）的遗传多样性，以制定作物改良策略

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Isabgol (Plantago ovata?F.,?family: Plantaginaceae)?is one of the most importantmedicinal plants of South Asia. Two DNA based molecular marker techniques, random amplified?polymorphic?DNA (RAPD) and inter-simple sequence repeat (ISSR), were used to study the genetic diversity among genotypes of Isabgol. A total of 38 polymorphic primers (22 random and 16 ISSR) were used. Amplification of genomic DNA of 24 genotypes, using RAPD analysis, yielded 208 fragments, of which 98 (47.12%) were polymorphic. The 16 ISSR primers produced 124 bands across 24 genotypes, of which 24 (19.35%) were polymorphic. RAPD markers appeared more informative than ISSR in determining the genetic diversity.?The similarity coefficient ranged from 0.77 to 0.97, 0.81 to 1.00 and 0.84 to 0.98 with RAPD, ISSR and combined dendrogram, respectively. This indicates very low level of genetic diversity among genotypes. A poor mantel correlation (r?=?0.28) was found between both sets of genetic similarity data, suggesting that both sets of markers revealed unrelated estimates of genetic relationships. Therefore, the RAPD and ISSR markers show two genetic grouping of studied Isabgol genotypes. The genotypes RI-168, RI-167, RI-137, PB-62, RI-153, RI-148 and Gumary were spotted as genetically diverse in both sets of markers and could be efficiently utilized in crop improvement programmes.
机译：Isabgol（Plantago ovata？F。，家族：Plantaginaceae）是南亚最重要的药用植物之一。研究了两种基于DNA的分子标记技术，即随机扩增多态性DNA（RAPD）和简单序列间重复（ISSR），以研究伊萨博尔各基因型之间的遗传多样性。总共使用了38个多态性引物（22个随机引物和16个ISSR）。使用RAPD分析，扩增24个基因型的基因组DNA，得到208个片段，其中98个片段（47.12％）是多态的。 16种ISSR引物在24个基因型中产生124条带，其中24条（19.35％）是多态性的。 RAPD标记在确定遗传多样性方面比ISSR更具参考价值。与RAPD，ISSR和组合树状图的相似系数分别为0.77至0.97、0.81至1.00和0.84至0.98。这表明基因型之间的遗传多样性水平非常低。在两组遗传相似性数据之间均发现了较差的壁炉架相关性（r？=？0.28），这表明两组标记都揭示了遗传关系的不相关估计。因此，RAPD和ISSR标记显示出已研究的伊萨博尔基因型的两个遗传分组。在两组标记中均发现了基因型RI-168，RI-167，RI-137，PB-62，RI-153，RI-148和Gumary的遗传多样性，可有效地用于作物改良计划。

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