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首页> 外文期刊>African Journal of Biotechnology >Molecular and pathological identification of feline coronavirus type I
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Molecular and pathological identification of feline coronavirus type I

机译：猫I型冠状病毒的分子和病​​理学鉴定

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The coronavirus in cats has been described as feline infectious peritonitis (FIPV) and feline enteric coronavirus (FECV). FIPV is highly fatal and caused immune-mediated pyogranulomatous disease, whereas FECV causes mild enteric infection. In this study, we described the isolation and molecular characterization of naturally occurring feline coronavirus from domestic cat in Malaysia. Additionally, the resultant pathological conditions observed in the infected cat were reported. Ascitic fluid sample was collected from 3-year-old domestic cat clinically suspected of effusive (wet form) FIP and subjected to virus isolation in?Felis catus?whole fetus cell cultures (Fcwf-4). The result of virus isolation was confirmed using one step reverse transcription polymerase chain reaction (RT-PCR).?To gain insight on the genetic variant of FCoV, the S-gene sequence was amplified using type 1 specific primers.Virus isolation showed cytopathic effect (CPE) characterized by giant cells, ballooning and cells detachment. Ultrastructural findings showed?virus particles attached to plasma membrane and later invaginated from the cell membrane. Virus-like particles were also observed in the vacuoles, likely as a result of spillage of mature virus-like particles into the cytoplasmic matrix.?Histopathological examination of kidney, spleen and intestine organ samples revealed coagulative necrosis with infiltration of neutrophils and mononuclear cells.?In conclusion, this study reports the first isolation of feline coronavirus in Malaysian cats and importantly the isolated virus was confirmed to be type I using S-gene amplification.
机译：猫中的冠状病毒已被描述为猫传染性腹膜炎（FIPV）和猫肠冠状病毒（FECV）。 FIPV致命，并引起免疫介导的脓性肉芽肿性疾病，而FECV引起轻度肠感染。在这项研究中，我们描述了来自马来西亚家猫的天然猫冠状病毒的分离和分子特征。另外，据报道在感染的猫中观察到的最终病理状况。从临床怀疑疑似散发性（湿性）FIP的3岁家猫中收集腹水样品，并在“猫属”全胎儿细胞培养物中（Fcwf-4）进行病毒分离。通过一步反转录聚合酶链反应（RT-PCR）证实了病毒分离的结果。为了深入了解FCoV的遗传变异，使用1型特异性引物扩增了S基因序列。病毒分离显示出细胞病变作用（CPE）的特征在于巨细胞，膨胀和细胞分离。超微结构发现，病毒颗粒附着在质膜上，随后从细胞膜上侵入。在液泡中还观察到病毒样颗粒，这可能是由于成熟的病毒样颗粒溢出到细胞质基质中所致。肾脏，脾脏和肠器官样品的组织病理学检查显示，中性粒细胞和单核细胞浸润性凝固性坏死。总而言之，这项研究报告了马来西亚猫首次分离出猫冠状病毒，重要的是，使用S基因扩增证实了分离出的病毒为I型。

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