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首页> 外文期刊>African Journal of Biotechnology >The sequence-related amplified polymorphism (SRAP) markers linked to the color around the stone (Cs) locus of peach fruit
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The sequence-related amplified polymorphism (SRAP) markers linked to the color around the stone (Cs) locus of peach fruit

机译：序列相关的扩增多态性（SRAP）标记与桃果实的石头（Cs）基因座周围的颜色相关

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摘要

The distribution and concentration of anthocyanin, a major subgroup of the flavonoids and important determinants of peach fruit flesh color, is regulated by several genes. In the international reference?Prunus?map, the locus of flesh color around the stone (Cs) is located on linkage group LG3. This report describes the development of a sequence-related amplified polymorphism (SRAP) marker linked to major genes controlling the?Cs?in peach. One F1 progeny from a cross between the peach cultivars ‘Chongyanghong’ × ‘Yanhong’ segregated by fruit flesh color around the stone was used for bulked segregant analysis. Two kinds of bulked DNA were constructed and used for the polymerase chain reaction in SRAP analysis. After 88 pairs of primers were screened against the two kinds of bulked DNA, three dominant SRAP markers were selected in cooperation with the?Cs?bulk. One of the three markers (Me07Em02) was linked to the?Cs?locus at a distance of 0 cM. The SRAP markers would provide an effective tool for marker-assisted selection for the trait of anthocyanin intensity of peach flesh in peach breeding programs.
机译：花青素是类黄酮的主要亚类，是桃果肉颜色的重要决定因素，其分布和浓度受几个基因调控。在国际参考“李子”图中，石头（Cs）周围的肉色轨迹位于连锁群LG3上。该报告描述了与桃子中控制Css的主要基因相关的序列相关扩增多态性（SRAP）标记的发展。桃子品种“重阳红”ד延红”的杂交后代中的一个F1后代被石头周围的果肉颜色分隔，用于大量分离物分析。构建了两种扩增的DNA，并将其用于SRAP分析中的聚合酶链反应。在针对这两种扩增的DNA筛选88对引物后，与？Cs？bulk一起选择了三个优势SRAP标记。三个标记之一（Me07Em02）以0 cM的距离与Cs？基因座连锁。 SRAP标记将为桃育种中桃肉花色苷强度特征的标记辅助选择提供有效的工具。

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