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首页> 外文期刊>African Journal of Food, Agriculture, Nutrition and Development >Adaptation of glass columns for clean-up RP-HPLC determination of aflatoxins with post-column derivatisation with bromine and fluorescence detection.
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Adaptation of glass columns for clean-up RP-HPLC determination of aflatoxins with post-column derivatisation with bromine and fluorescence detection.

机译：色谱柱后溴化和荧光检测，适用于玻璃柱净化RP-HPLC测定黄曲霉毒素。

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Consumption of aflatoxin-contaminated foods in developing countries may not only cause cancer in the general population, but is also associated with stunting and immune suppression in children. Accurate determination of aflatoxins in foods is important for the prevention of the above health risks. This study aimed to evaluate the possibility of using cheap florisil-packed glass columns instead of relatively complex and expensive pre-packed cartridges for afltoxin clean-up prior to HPLC analysis. Additionally, effects of aflatoxin extract storage temperature, and the moisture content of florisil on aflatoxin recovery were assessed. Results showed that the standard curve was highly linear as depicted by the high correlation coefficient for all four aflatoxins [0.99, 0.99, 0.97 and 0.98, respectively for AFG1, AFB2 and AFB1 and AFG2. The retention times over a total run time of 20 minutes were 14.8 (SD 0.4), 12.4 (SD 0.1), 10.7 (SD 0.3) and 9.1 (SD 0.3) minutes, respectively for AFB1, AFB2, AFG1 and AFG2. Florisil powder that had been dried for 12 hours at 100 oC, then hydrated with water at 5% (w/v) before packing on glass columns gave slightly higher recovery for all aflatoxins (100.3% AFB1, 93.1% AFB2, 83.8% AFG1 and 71.1% AFG2) compared to the pre-packed cartridges (96.4% AFB1, 91.5% AFB2, 80.6% AFG1 and 66.9% AFG2). There was 10% and 20% aflatoxin loss at 4 oC and 22 oC, respectively. After 6 hours of storage, no aflatoxin degradation was observed at – 18 oC. There was 0-5% aflatoxin loss at –18 oC after 12 hours, while at 4 oC and 22 oC, the loss range was 20-50% and 30-55%, respectively. Aflatoxin recoveries ranged from 120-160%, 90-110%, 80-98%, and 55-74% for florisil which had been hydrated with water at 2.5%, 5% and 10%, respectively. Glass columns are cheap and can be used many times and are a feasible and sustainable alternative to pre-packed florisil cartridges for aflatoxin clean up to ensure food safety, especially in resource poor settings.
机译：在发展中国家食用受黄曲霉毒素污染的食物不仅可能导致普通人群罹患癌症，而且还可能导致儿童发育迟缓和免疫抑制。准确测定食品中的黄曲霉毒素对预防上述健康风险很重要。这项研究的目的是评估在进行HPLC分析之前，使用廉价的装有弗里西尔的玻璃柱代替相对复杂且昂贵的预装柱子进行黄曲霉毒素清除的可能性。此外，评估了黄曲霉毒素提取物的储存温度和弗洛里西尔的水分含量对黄曲霉毒素回收率的影响。结果表明，标准曲线是高度线性的，正如所有四种黄曲霉毒素的高相关系数所表示的[AFG1，AFB2，AFB1和AFG2分别为0.99、0.99、0.97和0.98。对于AFB1，AFB2，AFG1和AFG2，在20分钟总运行时间内的保留时间分别为14.8（SD 0.4），12.4（SD 0.1），10.7（SD 0.3）和9.1（SD 0.3）分钟。将Florisil粉末在100 oC下干燥12小时，然后以5％（w / v）的水合，然后填充到玻璃柱上，对所有黄曲霉毒素（100.3％AFB1、93.1％AFB2、83.8％AFG1和AFG2为71.1％），而预装墨盒（AFB1为96.4％，AFB2为91.5％，AFG1为80.6％和AFG2为66.9％）。在4 oC和22 oC时黄曲霉毒素损失分别为10％和20％。储存6小时后，在– 18 oC下未观察到黄曲霉毒素降解。 12小时后，–18 oC，黄曲霉毒素损失为0-5％，而在4 oC和22 oC，黄曲霉毒素的损失范围分别为20-50％和30-55％。分别以2.5％，5％和10％的水水合的弗洛里西尔的黄曲霉毒素回收率范围为120-160％，90-110％，80-98％和55-74％。玻璃柱价格便宜，可多次使用，是预先包装的弗洛西尔药筒的一种可行且可持续的替代品，用于清理黄曲霉毒素以确保食品安全，特别是在资源贫乏地区。

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