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首页> 外文期刊>African Journal of Biotechnology >Interspecies embryo reconstruction in Tibetan antelope Pantholops hodgsonii by handmade cloning
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Interspecies embryo reconstruction in Tibetan antelope Pantholops hodgsonii by handmade cloning

机译：手工克隆法重建藏羚羊霍奇森种间胚胎

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摘要

Interspecies somatic cell nuclear transfer (iSCNT) offers the possibility of preserving endangered species. Handmade cloning (HMC) has proved to be an efficient alternative to the traditional micromanipulator-based technique in some domestic animal species. This study investigates the possibility of reconstructing the embryos of Tibetan antelope?Pantholops hodgsoni?by HMC and reports the development of cloned embryos. Fibroblast cells derived from ear tissue from a female Tibetan antelope 3 h after death were used as nuclear donors, and iSCNT was performed using goat oocytes matured?in vitro?as recipients. A total of 118 embryos were produced by HMC with a cleavage rate of 70.14% and morula formation rate of 100%. However, no blastocysts were obtained?in vitro. These results show that somatic cell nuclei of Tibetan antelope can be reprogrammed by goat oocytes. We also demonstrate that HMC is an efficient method of iSCNT using Tibetan antelope nuclear donors. Although the reconstructed embryos did not develop into blastocysts, the morula can be transferred into the womb of a recipient to continue development. Thus, we established an efficient method of iSCNT for producing transplantable embryos in Tibetan antelope by HMC.
机译：种间体细胞核转移（iSCNT）提供了保存濒危物种的可能性。在某些家畜中，手工克隆（HMC）已被证明是基于传统微操纵器技术的有效替代品。本研究探讨了通过HMC重建藏羚羊“ Pantholops hodgsoni”胚胎的可能性，并报道了克隆胚胎的发育。死后3小时从雌性藏羚羊的耳朵组织中获得的成纤维细胞用作核供体，并使用体外成熟的山羊卵母细胞作为受体进行iSCNT。 HMC共产生118个胚胎，卵裂率70.14％，桑ula形成率100％。但是，在体外没有获得胚泡。这些结果表明，藏羚羊的体细胞核可以被山羊卵母细胞重编程。我们还证明，HMC是使用藏羚羊核供体的iSCNT的有效方法。尽管重建的胚胎没有发育成胚泡，但桑可以被转移到接受者的子宫中以继续发育。因此，我们建立了一种有效的iSCNT方法，用于通过HMC生产藏羚羊的可移植胚胎。

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