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首页> 外文期刊>African Journal of Biotechnology >Occurrence and detection of extended-spectrum -lactamases in Klebsiella isolates in Hilla, Iraq
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Occurrence and detection of extended-spectrum -lactamases in Klebsiella isolates in Hilla, Iraq

机译：伊拉克希拉克雷伯菌分离株中广谱内酰胺酶的发生和检测

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A total of 88?K.?pneumoniae?strains were?isolated from different environmental and clinical samples in Hilla/Iraq during the period from January to July 2003.?Primary screening of β-Lactam resistant isolates showed that 73.8% (65?strains) were resistant to β-Lactam antibiotics. 58.4% of these strains were β-lactamase-producers. All the β- lactamase-producing?Klebsiella?strains showed multiple-drug resistance to least 8 antibiotics.?Klebsiella?strains were also tested for their ability to produce extended-spectrum beta-lactamases (ESBLs) using three methods. Determination of minimum inhibitory concentration (MIC) with and without clavulanate was the most accurate method for detection of ESBL-producing isolates, by which 8 (21%) ESBL-producing isolates were detected. Plasmid profile of?Klebsiella?strains (including ESBL-producers) was detected. The genes encoding for the production of ESBLs and resistance to penicillin, ampicillin, amoxicillin, tetracycline, refampin, and erythromycin were located on conjugative plasmids whereas genes encoding for resistance to cephalothin, cefazolin, cephalexin and gentamycin were located on the chromosome.
机译：在2003年1月至7月期间，从Hilla /伊拉克的不同环境和临床样品中共分离出88株K.p.肺炎克雷伯菌。初步筛选出耐β-内酰胺的分离株，发现73.8％（65株）。 ）对β-内酰胺抗生素有抗药性。这些菌株中58.4％是β-内酰胺酶生产者。所有产生β-内酰胺酶的“克雷伯菌”菌株均显示出对至少8种抗生素的多重耐药性。还使用三种方法测试了“克雷伯氏菌”菌株产生广谱β-内酰胺酶（ESBLs）的能力。有或没有克拉维酸根的最低抑菌浓度（MIC）的测定是检测ESBL产生菌株的最准确方法，可检测到8种（21％）ESBL产生菌株。检测了克雷伯氏菌菌株（包括ESBL生产者）的质粒图谱。编码ESBLs和对青霉素，氨苄青霉素，阿莫西林，四环素，再霉素和红霉素有抗性的基因位于接合质粒上，而对头孢菌素，头孢唑林，头孢氨苄和庆大霉素有抗性的基因则位于染色体上。

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