首页> 外文会议>2011 International Conference on Human Health and Biomedical Engineering >Investigation on CTX-M-type extended-spectrum (β-lactamase of Klebsiella pneumonia in Jilin Urban Area
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Investigation on CTX-M-type extended-spectrum (β-lactamase of Klebsiella pneumonia in Jilin Urban Area

机译:吉林市肺炎克雷伯菌CTX-M型超广谱(β-内酰胺酶)的研究

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To investigate the subtype of CTX-M-type extended-spectrum β-lactamase encoding gene and to research the resistance of Klebsiella pneumonia in Jilin Urban Area, and analysis the epidemiological aspect of CTX-M-producing isolates. PCR was used to detect CTX-M encoding gene in 118 strains of Klebsiella pneumoniae. Disc diffusion method was used to determine 14 kinds of antibiotics resistance for positive isolates. Arbitrarily primed polymerase chain reaction (AP-PCR) technique was used to type CTX-M producing isolates, and the fingerprinting profiles were produced according to the number and size of DNA bands. PCR amplification of 118 strains, 25 strains with CTX-M genotypes, account for 21.19%; which CTX-M-1 of 13 (11.02%); CTX-M-9 of 16 (13.56%); both with CTX-M-1 and CTX-M-9 of 4 strains (3.39%); CTX-M-2, CTX-M-8 and the CTX-M-25 were negative. 25 CTX-M positive strains were multidrug resistant (≥=53 antibiotics). The highest serviceability ratio of bacteria is in turn erythromycin (100%), cefotaxime (92%), ampicillin (92%), tetracyclin (88%). The lower rate of imipenem (4%) was susceptible to meropenem. There were 13 RAPD types in 26 isolates of 25 strains of CTX-M positive bacteria. CTX-M type extended spectrum β-lactamases of Klebsiella pneumoniae as multiple drug-resistant bacteria, CTX-M enzymes are certainly colony spreading.
机译:研究吉林市区CTX-M型超广谱β-内酰胺酶编码基因的亚型,研究其对肺炎克雷伯菌的抵抗力,并分析产生CTX-M的分离株的流行病学特征。 PCR用于检测118株肺炎克雷伯菌的CTX-M编码基因。采用圆盘扩散法测定阳性分离株对14种抗生素的耐药性。使用任意引发的聚合酶链反应(AP-PCR)技术对产生CTX-M的菌株进行分型,并根据DNA条带的数量和大小产生指纹图谱。 PCR扩增118株,CTX-M基因型25株,占21.19%;其中CTX-M-1为13(11.02%); CTX-M-9为16(13.56%); CTX-M-1和CTX-M-9均为4株(3.39%); CTX-M-2,CTX-M-8和CTX-M-25为阴性。 25例CTX-M阳性菌株具有多重耐药性(≥= 53种抗生素)。细菌的最高使用率依次是红霉素(100%),头孢噻肟(92%),氨苄青霉素(92%),四环素(88%)。亚胺培南的较低比率(4%)易受美罗培南的影响。在25株CTX-M阳性细菌的26株菌株中,有13种RAPD类型。肺炎克雷伯菌的CTX-M型超广谱β-内酰胺酶是多种耐药菌,CTX-M酶肯定在菌落中扩散。

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