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Effect of the neurosphere size on the viability and metabolism of neural stem/progenitor cells

机译：神经球大小对神经干/祖细胞活力和代谢的影响

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The objective of this study?was?to investigate the metabolic activity and viability of the mouse neural stem/progenitor cells (NSPCs) affected by the size of neurospheres. NSPCs dissociated from the forebrain of embryonic 14 days (E14) mice were cultured in flask for 120 h. During cultivation, the diameter distribution of neurospheres, cell viability and metabolic activities were monitored, together with the concentrations of glucose, lactate, glutamine and ammonia in the media. The results show that cell activity decreased with?the?increment of neurospheres size. When the diameter reached about 100 μm and the concentration of glucose and glutamine were 36.38 and 1.33 mmol/L, the growth of central cells in neurospheres began to surface. Furthermore, when the diameter reached about 100 to 150 μm and the concentrations of glucose and glutamine were 31.11 and 1.15 mmol/L, simultaneously, the death rate of NSPCs was larger than that of the newly born cells within the neurospheres. The metabolic activity of the cells declined to a very low level. This observation can be explained by diffusion limitation of nutrients and metabolic waste inside neurosphere. In conclusion, the mass transfer will be limited when the neurospheres size reaches a critical value of 100 to 150 μm and beyond this critical value, serious impact of nutrient supply and metabolites on the cell viability and metabolism occurs.
机译：这项研究的目的是调查受神经球大小影响的小鼠神经干/祖细胞（NSPC）的代谢活性和生存能力。从胚胎的14天前（E14）的前脑解离的NSPCs在烧瓶中培养120 h。培养期间，监测神经球的直径分布，细胞活力和代谢活性，以及​​培养基中葡萄糖，乳酸，谷氨酰胺和氨的浓度。结果表明，细胞活性随神经球大小的增加而降低。当直径达到约100μm时，葡萄糖和谷氨酰胺的浓度分别为36.38和1.33 mmol / L，神经球中央细胞的生长开始浮出水面。此外，当直径达到约100至150μm且葡萄糖和谷氨酰胺的浓度分别为31.11和1.15 mmol / L时，NSPCs的死亡率要比神经球内新生细胞的死亡率高。细胞的代谢活性下降到非常低的水平。该观察结果可以通过营养物质和代谢废物在神经球内部的扩散限制来解释。总之，当神经球大小达到100至150μm的临界值且超过该临界值时，传质将受到限制，营养物供应和代谢物对细胞生存力和代谢的严重影响会发生。

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