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首页> 外文期刊>African Journal of Biotechnology >Characterization of Metarhizium anisopliae using amplifed ribossomal DNA restriction analysis (ARDRA) and internal transcribed spacer (ITS) sequence analysis
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Characterization of Metarhizium anisopliae using amplifed ribossomal DNA restriction analysis (ARDRA) and internal transcribed spacer (ITS) sequence analysis

机译：使用扩增的核糖体DNA限制分析（ARDRA）和内部转录间隔区（ITS）序列分析来表征间质异形体

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Molecular methods have revolutionized systematic entomology in the genusMetarhizium. In Brazil, the importance of these entomopathogenic fungi in agriculture is critical to the control of the sugar cane spittlebug (Mahanarva posticata) (Homoptera: Cercopidae). Other important factors for the selection of isolates ofMetarhizium anisopliae?for use in the biological control of particular pests are virulence, reproductive characteristics and the media of artificial cultures. In addition, the genetic variability of these entomopathogenic fungi indicates a great potential for pest control and a lack of a link between?isolates from specific host sites?and?fungal virulence. We studied five?Metarhizium?hosts including the sugar cane spittlebug (IPA213, IPA215, IPA219 and IPA216) and the grasshopper (IPA217).?It is noteworthy that these five isolates are used as biological control for growing sugar cane on an industrial scale for the Brazilian Northeast. Hence, the importance to genetically characterize these isolates of?Metarhizium?cannot be over emphasized.?Amplifed ribossomal DNA restriction analysis (ARDRA) did not differentiate any of the isolates that were studied following digestion with three endonuclease namely;?HindIII,?HinfI and?HpaI. The data that were generated using ITS1 and ITS4 markers revealed that although these isolates infect the same host, they are genetically distinct?perhaps due to the unknown origin of the hosts. However, the isolate IPA217, which was isolated from the grasshopper, did not behave differently from the others. These results show that the?M. anisopliae?complex is monophyletic and it was concluded that the?use of markers and morphological studies yielded results that may corroborate the idea that investigation of the phenotypic and molecular characteristics of this genus may indentify a new species of?Metarhizium.
机译：分子方法彻底改变了金属甲虫属的系统昆虫学。在巴西，这些昆虫病原真菌在农业中的重要性对于控制甘蔗的臭虫（Mahanarva posticata）（Homoptera：Cercopidae）至关重要。选择用于对特定有害生物进行生物防治的金属甲硫菌的分离株的其他重要因素是毒力，繁殖特性和人工培养的培养基。此外，这些昆虫病原真菌的遗传变异性表明，其具有很大的控制病虫害的潜力，并且缺乏来自特定宿主位点的分离物与真菌毒力之间的联系。我们研究了五种“ Metarhizium”寄主，包括甘蔗的臭虫（IPA213，IPA215，IPA219和IPA216）和蚱hopper（IPA217）。值得注意的是，这五种分离物被用作工业规模上种植甘蔗的生物防治。巴西东北。因此，不能过分强调对这些金属分离基因的重要性。扩增的核糖体DNA限制性内切酶分析（ARDRA）并没有区分用三种内切核酸酶（即：HindIII，HinfI和？HpaI。使用ITS1和ITS4标记生成的数据表明，尽管这些分离株感染同一宿主，但由于宿主的来源未知，它们在遗传上可能不同。但是，从蝗虫中分离出的分离株IPA217的行为与其他人没有什么不同。这些结果表明。 anisopliae？复合体是单系的，因此得出结论，使用标记和形态学研究得出的结果可能证实了这一属的表型和分子特征的研究可能会鉴定出新的Metarhizium的观点。

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