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首页> 外文期刊>American Journal of Drug Discovery and Development >Antimicrobial Resistance and Plasmid Characterization of Escherichia coli Isolated in In natura Water
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Antimicrobial Resistance and Plasmid Characterization of Escherichia coli Isolated in In natura Water

机译:自然水中分离的大肠杆菌的抗药性和质粒鉴定

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The constant use of antibiotics in human and animal medicines is causing a rise in Escherichia coli resistance to these medicines, interfering with the effective treatment of infections caused by this agent. For this study, we isolated and identified E. coli from in nature water samples and verified the presence of plasmids that caused bacteria resistance. We collated 24 water samples from two different stations (a water treatment station and a water captation station). From the contaminated samples, we evaluated antibiotic resistance to amoxicillin (5 μg) cephalexin (5 μg), azitromicin (5 μg), ampicilin (5 μg), tetracycline (5 μg) and ciprofloxacin (10 μg). We also evaluated whether the resistance was plasmidial or chromosomal. The results showed a frequent occurrence of multiple resistance to the main antimicrobials utilized, includingcephalexin (67.44%), amoxicilin (62.79%), ampicilin (58.13%), tetracycline (37.20%), azithromycin (32.55%), ciproflaxacin (18.60%). We observed the presence of different plasmidial profiles, including occurrences of amoxicillin (30%), ampicillin (30%), tetracycline (30%) and ciprofloxacin (10%). The study showed that the samples presented plasmids with genes resistant to important antibiotics used for public health.
机译:在人类和动物药物中不断使用抗生素会导致大肠杆菌对这些药物的耐药性上升,从而干扰了对该药物引起的感染的有效治疗。对于本研究,我们从自然水样中分离并鉴定了大肠杆菌,并验证了引起细菌抗性的质粒的存在。我们从两个不同的站点(一个水处理站和一个水质鉴定站)整理了24个水样。从受污染的样品中,我们评估了对阿莫西林(5μg)头孢氨苄(5μg),阿奇霉素(5μg),氨苄青霉素(5μg),四环素(5μg)和环丙沙星(10μg)的抗生素抗性。我们还评估了抗性是质粒的还是染色体的。结果表明,对所用主要抗菌药物的多重耐药性频繁发生,包括头孢氨苄(67.44%),阿莫西林(62.79%),氨苄青霉素(58.13%),四环素(37.20%),阿奇霉素(32.55%),环丙沙星(18.60%) 。我们观察到存在不同的质粒谱,包括阿莫西林(30%),氨苄青霉素(30%),四环素(30%)和环丙沙星(10%)的出现。研究表明,样品中的质粒具有对用于公共卫生的重要抗生素具有抗性的基因。

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