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首页> 外文期刊>American journal of food technology >Effect of Sample Pre-enrichment and Characters of Food Samples on the Examination for the Salmonella by Plate Count Method and Fluorescent in-situ Hybridization Technique
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Effect of Sample Pre-enrichment and Characters of Food Samples on the Examination for the Salmonella by Plate Count Method and Fluorescent in-situ Hybridization Technique

机译：平板计数法和荧光原位杂交技术对样品预富集和食品样品特性对沙门氏菌检测的影响

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摘要

In most of the novel and traditional methods used in the examination of food samples for Salmonella, pre-enrichment of samples is used as a means of increasing the sensitivity and reliability. However, the influence of pre-enrichment of sample on some of those methods has not been studied. Furthermore, the effect of the conditions of the sample on the sensitivity of some of those methods are also not been studied. The aim of this research was to study the influence of pre-enrichment and the conditions of the samples on the detection sensitivity of one novel method Fluorescent in-situ Hybridization (FISH) and one traditional culture techniques (XLT-4 agar plates). To study the influence of enrichment, 60 pork sausage samples collected from 20 different food outlets were examined for Salmonella contamination by both methods with and without pre-enrichment. To study the effect of the conditions of food, collected samples were spiked with 107 cfu mL-1 Salmonella enterica culture and examined with and without pre-enrichment. Detection sensitivity of both methods was higher in pre-enriched fresh samples as well as spiked samples. FISH method was found to be more sensitive and less affected by the conditions of food, compared to culture method.
机译：在用于食品样品中沙门氏菌检查的大多数新颖和传统方法中，样品的预富集被用作提高灵敏度和可靠性的一种手段。但是，尚未研究样品预富集对其中一些方法的影响。此外，还没有研究样品条件对这些方法中某些方法的敏感性的影响。这项研究的目的是研究一种新方法荧光原位杂交（FISH）和一种传统培养技术（XLT-4琼脂平板）的预富集和样品条件对检测灵敏度的影响。为了研究富集的影响，采用预富集和不富集的两种方法对从20个不同食品商店收集的60个猪肉香肠样品进行了沙门氏菌污染检测。为了研究食物条件的影响，将收集的样品掺入10 7 cfu mL -1 肠沙门氏菌培养物，并进行有无预富集的检查。在预富集的新鲜样品和加标样品中，两种方法的检测灵敏度均较高。与培养方法相比，发现FISH方法更加敏感，受食物条件的影响较小。

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