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Salmonella Testing of Pooled Pre-Enrichment Broth Cultures for Screening Multiple Food Samples

机译：沙门氏菌混合培养前富集肉汤培养物中多种食品样品的筛选

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摘要

A method has been described for testing multiple food samples for Salmonella without loss in sensitivity. The method pools multiple pre-enrichment broth cultures into single enrichment broths. The subsequent stages of the Salmonella analysis are not altered. The method was found applicable to several dry food materials including nonfat dry milk, dried egg albumin, cocoa, cottonseed flour, wheat flour, and shredded coconut. As many as 25 pre-enrichment broth cultures were pooled without apparent loss in the sensitivity of Salmonella detection as compared to individual sample analysis. The procedure offers a simple, yet effective, way to increase sample capacity in the Salmonella testing of foods, particularly where a large proportion of samples ordinarily is negative. It also permits small portions of pre-enrichment broth cultures to be retained for subsequent individual analysis if positive tests are found. Salmonella testing of pooled pre-enrichment broths provides increased consumer protection for a given amount of analytical effort as compared to individual sample analysis.
机译：已经描述了一种用于测试多个食品样品中沙门氏菌而不降低灵敏度的方法。该方法将多个预富集肉汤培养物合并为单个富集肉汤。沙门氏菌分析的后续阶段没有改变。发现该方法适用于几种干食品原料，包括脱脂奶粉，蛋清蛋白干，可可，棉籽粉，小麦粉和椰丝。与单个样品分析相比，汇集了多达25种预富集肉汤培养物，沙门氏菌检测的灵敏度没有明显下降。该程序提供了一种简单而有效的方法来提高食品在沙门氏菌检测中的样品容量，尤其是在大部分样品通常为阴性的情况下。如果发现阳性试验，它还可以保留一小部分的浓缩前肉汤培养物，以便随后进行单独分析。与给定的样品分析相比，沙门氏菌对浓缩的预富集肉汤的检测可在给定的分析工作量下为消费者提供更多保护。

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