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首页> 外文期刊>Analytical methods >A multi-element stable isotope approach coupled with chemometrics for the determination of Tieguanyin tea geographical origin and harvest season
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A multi-element stable isotope approach coupled with chemometrics for the determination of Tieguanyin tea geographical origin and harvest season

机译：多元素稳定同位素方法与化学计量学结合用于测定铁观音茶的地理来源和收获季节

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The taste and aroma quality and the price of tea are largely impacted by the geographical origin and harvest season. Tieguanyin teas differing in sites of growth and harvest season were characterized by the analysis of stable isotopes of nitrogen, carbon, hydrogen and oxygen (δ15N, δ13C, δD and δ18O). The δ15N, δ13C, δD and δ18O values were determined by elemental analyzer-isotope ratio mass spectrometry (EA-IRMS). All δ15N, δ13C, δD and δ18O values exhibited significant differences (p 0.05) in the samples of different regional origins, whereas the δD and δ18O values highly differed (p 0.01) between spring and autumn Tieguanyin teas. With the aid of chemometrics, orthogonal projection to latent structures discriminate analysis (OPLS-DA) achieved 96.08% recognition rate for Tieguanyin teas from different regions, whereas stepwise linear discriminant analysis (SLDA) allowed 100% differentiation. Moreover, principal component analysis (PCA) and hierarchical cluster analysis (HCA) methods revealed excellent discrimination possibilities for the teas according to seasonal variations. Additionally, our study presented a good alternative to differentiate teas from different sites of growth and harvest seasons and also to address the quality control and food authenticity concerns.
机译：茶的味道和香气质量以及价格在很大程度上受地理来源和收获季节的影响。通过分析氮，碳，氢和氧的稳定同位素（δ15N，δ13C，δD和δ18O）来表征在生长和收获季节不同的铁观音茶。 δ15N，δ13C，δD和δ18O值通过元素分析仪-同位素比质谱（EA-IRMS）确定。铁观音茶与秋季铁观音茶的所有δ15N，δ13C，δD和δ18O值均表现出显着差异（p <0.05），而δD和δ18O值则差异很大（p <0.01）。借助化学计量学，对潜在结构的正交投影判别分析（OPLS-DA）对来自不同地区的铁观音茶获得了96.08％的识别率，而逐步线性判别分析（SLDA）允许100％的区别。此外，主成分分析（PCA）和层次聚类分析（HCA）方法揭示了根据季节变化对茶进行出色区分的可能性。此外，我们的研究提出了一个很好的选择，可以区分来自生长和收获季节不同地点的茶，还可以解决质量控制和食品真实性方面的问题。

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