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Electrochemical DNA Detection Using Supramolecular Interactions

机译：使用超分子相互作用的电化学DNA检测

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An electrochemical DNA detection format has been developed to allow simple and rapid assays with downsized instruments. Since naphthalene diimide derivatives are known to bind to double stranded DNA (dsDNA) with threading intercalation, they are stabilized by pseudo-catenane formation. For example, ferrocenylnaphthalene diimide (FND) can be efficiently concentrated on dsDNA, thus enabling its electrochemical detection. Since dsDNA is formed between single stranded target DNA and DNA probe, the target DNA can be detected electrochemically, using DNA probe-immobilized on the electrode. Further stabilization of naphthalene diimide derivatives as a complex with dsDNA on the electrode is expected to lead to more precise and selective detection of target DNA. This approach was realized by the formation of inclusion complexes of ferrocene with β-cyclodextrin (β-CD) for the FND-dsDNA complexes on the electrode. Combination of adamantylnaphthalene diimide (AND) and ferrocenyl-β-CD (Fc-CD) gave new supramolecular DNA detection assays coupled with DNA probe-immobilized electrodes. The electrochemical signal increased only upon formation of the Fc-CD/AND complex bound to dsDNA on the electrode. Naphthalene diimide carrying ferrocene and β-CD realized “signal on” type detection of dsDNA in homogenous solution. Naphthalene diimide carrying two dithiolene termini provided a new method to immobilize dsDNA on the gold electrode that may be suitable for the electrochemical detection of target DNA.
机译：已经开发出电化学DNA检测形式，以允许使用小型仪器进行简单，快速的测定。由于已知萘二酰亚胺衍生物通过穿线插入结合到双链DNA（dsDNA），因此它们通过伪联烷形成而得以稳定。例如，二茂铁基萘二酰亚胺（FND）可以有效地浓缩在dsDNA上，从而使其能够进行电化学检测。由于dsDNA是在单链靶DNA和DNA探针之间形成的，因此可以使用固定在电极上的DNA探针以电化学方式检测靶DNA。萘二酰亚胺衍生物与dsDNA的复合物在电极上的进一步稳定有望导致对靶DNA的更精确和选择性的检测。通过在电极上形成二茂铁与β-环糊精（β-CD）的FND-dsDNA络合物的包合物来实现此方法。金刚烷基萘二酰亚胺（AND）和二茂铁基-β-CD（Fc-CD）的组合提供了新的超分子DNA检测方法，并结合了固定有DNA探针的电极。电化学信号仅在电极上结合dsDNA的Fc-CD / AND复合物形成后增加。带有二茂铁和β-CD的萘二酰亚胺实现了在均相溶液中dsDNA的“信号开启”型检测。带有两个二硫代苯末端的萘二酰亚胺提供了一种将dsDNA固定在金电极上的新方法，该方法可能适用于目标DNA的电化学检测。

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