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首页> 外文期刊>Annual Review & Research in Biology >Stimulation of Allogenic lymphocytes by Dendritic Cells Derived from Human Umbilical Cord Blood Fused with Breast Cancer Stem Cells
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Stimulation of Allogenic lymphocytes by Dendritic Cells Derived from Human Umbilical Cord Blood Fused with Breast Cancer Stem Cells

机译：人脐带血与乳腺癌干细胞融合的树突状细胞刺激同种异体淋巴细胞。

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Aims: Cancer stem cells (CSCs) are cancer cells that possess characteristics associated with normal stem cells. CSCs represent a minor subset of cells in the tumor and are thought to be the reason for the initiation of disease, resistance to cancer treatment, and the occurrence of metastasis. Therefore, breast cancer stem cells (BCSCs) targeting therapies are considered as the promising therapy for breast cancer treatment. This research aims to evaluate the tumor associated antigens presentation of dendritic cells fused with breast cancer stem cells in dendritic cells based therapies. Methodology: Human breast cancer stem cells are isolated from malignant breast tumors by enrich culture and fluorescent activated cell sorting. Human dendritic cells are isolated from umbilical cord blood by culturing CD14 monocytes in induced medium. Electrocution is used to fuse breast cancer stem cells and dendritic cells. Fusion cells are used to evaluate functions of dendritic cells (DCs) and also to stimulate T cells. Results: These findings indicate that fusion cells have the ability to present the antigens of breast cancer stem cell to T-cells, and regarding functionality.Conclusion: They appear to be very good candidates for antitumor vaccine in breast cancer.
机译：目的：癌症干细胞（CSC）是具有与正常干细胞相关的特征的癌细胞。 CSC代表肿瘤细胞中的一小部分，被认为是引发疾病，抗癌治疗和发生转移的原因。因此，靶向乳腺癌干细胞（BCSC）的疗法被认为是乳腺癌治疗的有前途的疗法。这项研究旨在评估树突状细胞与乳腺癌干细胞融合的树突状细胞的肿瘤相关抗原呈递方式。方法：通过富集培养和荧光激活细胞分选从恶性乳腺肿瘤中分离出人类乳腺癌干细胞。通过在诱导培养基中培养CD14单核细胞，从脐带血中分离人树突状细胞。电死刑用于融合乳腺癌干细胞和树突状细胞。融合细胞用于评估树突状细胞（DC）的功能并刺激T细胞。结果：这些发现表明融合细胞具有将乳腺癌干细胞的抗原呈递给T细胞以及有关功能的能力。结论：它们似乎是乳腺癌抗肿瘤疫苗的很好候选者。

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