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Magnetotactic bacterial cages as safe and smart gene delivery vehicles

机译：趋磁细菌笼作为安全和智能的基因传递载体

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In spite of the huge advances in the area of synthetic carriers, their efficiency still poorly compares to natural vectors. Herein, we report the use of unmodified magnetotactic bacteria as a guidable delivery vehicle for DNA functionalized gold nanoparticles (AuNPs). High cargo loading is established under anaerobic conditions (bacteria is alive) through endocytosis where AuNPs are employed as transmembrane proteins mimics (facilitate endocytosis) as well as imaging agents to verify and quantify loading and release. The naturally bio-mineralized magnetosomes, within the bacteria, induce heat generation inside bacteria through magnetic hyperthermia. Most importantly after exposing the system to air (bacteria is dead) the cell wall stays intact providing an efficient bacterial vessel. Upon incubation with THP-1 cells, the magnetotactic bacterial cages (MBCs) adhere to the cell wall and are directly engulfed through the phagocytic activity of these cells. Applying magnetic hyperthermia leads to the dissociation of the bacterial microcarrier and eventual release of cargo.
机译：尽管合成载体领域取得了巨大进步，但与天然载体相比，它们的效率仍然很差。在本文中，我们报道了未修饰的趋磁细菌作为DNA功能化金纳米粒子（AuNPs）的有指导意义的传递载体的用途。在无氧条件下（细菌是活的），通过内吞作用（AuNPs被用作跨膜蛋白模拟物（促进内吞作用））以及显像剂来验证和量化装载和释放，从而建立了高货物装载量。细菌中的天然生物矿化磁小体通过磁热疗诱导细菌内部的热量产生。最重要的是，将系统暴露于空气中（细菌死亡）后，细胞壁将保持完整，从而提供有效的细菌容器。与THP-1细胞孵育后，趋磁细菌笼（MBC）粘附在细胞壁上，并通过这些细胞的吞噬活性直接被吞噬。施加磁热疗法会导致细菌微载体解离并最终释放货物。

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