• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Animal Biology Animal Husbandry-International Journal of the Bioflux Society >Mitochondrial CO1 gene sequence variation and the taxonomic status of three Macrobrachium species in Nigeria.
【24h】

Mitochondrial CO1 gene sequence variation and the taxonomic status of three Macrobrachium species in Nigeria.

机译：尼日利亚3个沼虾的线粒体CO1基因序列变异和分类状态。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Macrobrachium species are ubiquitous being found all over the world except in Europe. Theyare the most speciose of the decapods showing very great variations in response to the prevailingenvironmental conditions. This has led to serious difficulties with their classification based onmorphology. In Nigerian sympatric occurrences of Macrobrachium vollenhovenii, Macrobrachium dux andMacrobrachium macrobrachion have been reported in many water bodies. So far no record of a review ofthe morphological classification based on molecular markers have been reported. Sampling from fourmajor water bodies namely Warri, Badagry, Calabar and Asejire Dam all in Southern Nigeria was doneand mitochondria DNA marker CO1 genes were sequenced to determine the congruence of morphologicaland molecular classifications. A 556bp sequence was phylogenetically analyzed using the major methods.All the trees produced similar results. Instead the three species classification based on morphology,molecular phylogeny revealed consistently a two species grouping with M. vollenhovenii clearly mappingas a valid species. M. dux and M. macrobrachion were grouped together into one clade indicating a singlespecies or at best morphotypes of a species. Genetic diversity within and between the species is lowindicating a recent colonization process. The value of the bootstrap supporting the grouping was also low.Several primer combinations failed to amplify the CO1 gene of the Warri specimens. This is a quantitativemeasure of being distinct. The Nigerian Macrobrachium species maps closer to the Southern Americanspecies M. brasiliense, M. carcinus and M. olfersi. The Asian species M. asperulum and M. rosenbergii arethe most distant from the Nigerian Macrobrachium species. Further studies to reveal deeper phylogeneticstructuring using species specific primers may be needed to resolve fully the phylogenetic controversies.Determining the development patterns whether abbreviated or extended is also necessary. Thesequencing of the complete mitogenome is also an important further research requirement.
机译：除欧洲以外，在世界各地都发现了沼虾。它们是十足动物中最特殊的一种，对流行的环境条件有很大的变化。这给基于形态的分类带来了严重的困难。在尼日利亚，在许多水体中均报告有同伴发生的Vollenhovenii沼虾，dux沼虾和Macrobrachion沼虾。迄今为止，尚未有关于基于分子标记的形态学分类的综述的报道。从尼日利亚南部的沃里，巴达格里，卡拉巴尔和阿塞吉尔大坝四个主要水体取样，并对线粒体DNA标记CO1基因进行测序，以确定形态学和分子分类的一致性。使用主要方法对556bp序列进行了系统发育分析，所有树木均产生了相似的结果。取而代之的是，根据形态学，分子系统发育学对三个物种进行分类，发现一致地将两个物种与Vollenhovenii一起明确地映射为有效物种。杜氏支原体和巨支支原体被分组为一个进化枝，指示一个物种的单一物种或至多形态。物种内部和物种之间的遗传多样性低，表明最近的定殖过程。引导程序支持该分组的价值也很低。几种引物组合未能扩增Warri标本的CO1基因。这是区分的定量方法。尼日利亚Macrobrachium物种的地图更接近于南美物种巴西布拉斯朗霉菌，卡门氏菌和olfersi。亚洲种曲霉和罗氏沼虾与尼日利亚Macrobrachium种类最远。可能需要进行进一步的研究以揭示使用物种特异性引物进行更深入的系统发育构建，以完全解决系统发育争议。还需要确定发育模式的缩写或延伸。完整的有丝分裂基因组的排序也是重要的进一步研究要求。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号