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首页> 外文期刊>Antibiotics >In Vitro Antibiofilm Efficacies of Different Antibiotic Combinations with Zinc Sulfate against Pseudomonas aeruginosa Recovered from Hospitalized Patients with Urinary Tract Infection
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In Vitro Antibiofilm Efficacies of Different Antibiotic Combinations with Zinc Sulfate against Pseudomonas aeruginosa Recovered from Hospitalized Patients with Urinary Tract Infection

机译：从住院尿路感染患者中发现不同的抗生素组合与硫酸锌对铜绿假单胞菌的体外抗生物膜作用

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摘要

Urinary tract infections (UTIs) are a serious healthcare dilemma influencing millions of patients every year and represent the second most frequent type of body infection. Pseudomonas aeruginosa is a multidrug-resistant pathogen causing numerous chronic biofilm-associated infections including urinary tract, nosocomial, and medical devices-related infections. In the present study, the biofilm of P. aeruginosa CCIN34519, recovered from inpatients with UTIs, was established on polystyrene substratum and scanning electron microscopy (SEM) and was utilized for visualization of the biofilm. A previously described in vitro system for real-time monitoring of biofilm growth/inhibition was utilized to assess the antimicrobial effects of ciprofloxacin, levofloxacin, moxifloxacin, norfloxacin, ertapenem, ceftriaxone, gentamicin, and tobramycin as single antibiotics as well as in combinations with zinc sulfate (2.5 mM) against P. aeruginosa CCIN34519 biofilm. Meanwhile, minimum inhibitory concentrations (MICs) at 24 h and mutant prevention concentrations (MPCs) at 96 h were determined for the aforementioned antibiotics. The real-time monitoring data revealed diverse responses of P. aeruginosa CCIN34519 biofilm to the tested antibiotic-zinc sulfate combinations with potential synergisms in cases of fluoroquinolones (ciprofloxacin, levofloxacin, moxifloxacin, and norfloxacin) and carbapenem (ertapenem) as demonstrated by reduced MIC and MPC values. Conversely, considerable antagonisms were observed with cephalosporin (ceftriaxone) and aminoglycosides (gentamicin, and tobramycin) as shown by substantially increased MICs and MPCs values. Further deliberate in vivo investigations for the promising synergisms are required to evaluate their therapeutic potentials for treatment of UTIs caused by P. aeruginosa biofilms as well as for developing preventive strategies.
机译：尿路感染（UTI）是严重的医疗保健难题，每年影响数百万患者，是第二大常见的身体感染类型。铜绿假单胞菌是一种多药耐药病原体，可引起许多与生物膜相关的慢性感染，包括尿路，医院感染和医疗器械相关感染。在本研究中，铜绿假单胞菌CCIN34519的生物膜是在聚苯乙烯基质和扫描电子显微镜（SEM）上建立的，该膜是从UTI住院患者中回收的，并用于生物膜的可视化。利用先前描述的用于实时监测生物膜生长/抑制的体外系统，评估环丙沙星，左氧氟沙星，莫西沙星，诺氟沙星，厄他培南，头孢曲松，庆大霉素和妥布霉素作为单一抗生素的抗菌作用以及与锌的组合铜绿假单胞菌CCIN34519生物膜的硫酸盐（2.5 mM）。同时，对于上述抗生素测定了24小时的最小抑制浓度（MIC）和96小时的突变预防浓度（MPC）。实时监测数据显示，在氟喹诺酮类药物（环丙沙星，左氧氟沙星，莫西沙星和诺氟沙星）和碳青霉烯（ertapenem）的情况下，铜绿假单胞菌CCIN34519生物膜对所测试的抗生素-硫酸锌组合的多种反应具有潜在的协同作用。和MPC值。相反，对头孢菌素（头孢曲松）和氨基糖苷（庆大霉素和妥布霉素）的拮抗作用明显增加，MIC和MPC值明显升高。需要对有希望的增效作用进行进一步的体内研究，以评估其治疗由铜绿假单胞菌生物膜引起的尿路感染的治疗潜力以及制定预防策略。

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