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首页> 外文期刊>Antibiotics >Pectocin M1 (PcaM1) Inhibits Escherichia coli Cell Growth and Peptidoglycan Biosynthesis through Periplasmic Expression
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Pectocin M1 (PcaM1) Inhibits Escherichia coli Cell Growth and Peptidoglycan Biosynthesis through Periplasmic Expression

机译：果胶M1（PcaM1）通过周质表达抑制大肠杆菌细胞生长和肽聚糖生物合成

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摘要

Colicins are bacterial toxins produced by some Escherichia coli strains. They exhibit either enzymatic or pore-forming activity towards a very limited number of bacterial species, due to the high specificity of their reception and translocation systems. Yet, we succeeded in making the colicin M homologue from Pectobacterium carotovorum , pectocin M1 (PcaM1), capable of inhibiting E. coli cell growth by bypassing these reception and translocation steps. This goal was achieved through periplasmic expression of this pectocin. Indeed, when appropriately addressed to the periplasm of E. coli , this pectocin could exert its deleterious effects, i.e., the enzymatic degradation of the peptidoglycan lipid II precursor, which resulted in the arrest of the biosynthesis of this essential cell wall polymer, dramatic morphological changes and, ultimately, cell lysis. This result leads to the conclusion that colicin M and its various orthologues constitute powerful antibacterial molecules able to kill any kind of bacterium, once they can reach their lipid II target. They thus have to be seriously considered as promising alternatives to antibiotics.
机译：大肠菌素是由一些大肠杆菌菌株产生的细菌毒素。由于它们的接收和易位系统的高度特异性，它们对非常有限数量的细菌种类具有酶促或成孔活性。然而，我们成功地从胡萝卜杆菌，果胶M1（PcaM1）制备了大肠菌素M同源物，能够绕过这些接收和转运步骤来抑制大肠杆菌细胞的生长。该目标通过果胶的周质表达得以实现。的确，当果胶酶适当处理大肠杆菌的周质时，它可以发挥其有害作用，即肽聚糖脂质II前体的酶促降解，从而导致这种必需的细胞壁聚合物的生物合成被阻止，形态十分明显。变化，最终导致细胞裂解。该结果得出的结论是，大肠菌素M及其各种直系同源物构成了强大的抗菌分子，一旦它们能够达到脂质II靶标，它们就能杀死任何种类的细菌。因此，必须认真考虑它们是抗生素的有前途的替代品。

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