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首页> 外文期刊>Applications in plant sciences. >Genetic Variation at Microsatellite Loci in the Tropical Herb Aphelandra aurantiaca (Acanthaceae)
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Genetic Variation at Microsatellite Loci in the Tropical Herb Aphelandra aurantiaca (Acanthaceae)

机译：热带草本Aphelandra aurantiaca（棘皮科）中微卫星基因座的遗传变异

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Premise of the study: To assess the effect of forest fragmentation on genetic variation and population structure of Aphelandra aurantiaca (Acanthaceae), a tropical and ornamental herbaceous perennial plant, we developed the first microsatellite primers for the species. Methods and Results: Fourteen microsatellite markers were isolated and characterized from A. aurantiaca genomic libraries enriched for di-, tri-, and tetranucleotide repeat motifs. Polymorphism was evaluated in 107 individuals from four natural populations. Twelve out of 14 genetic markers were polymorphic. The number of alleles per locus ranged from two to 12, and the observed and expected heterozygosities ranged from 0.22 to 0.96 and from 0.20 to 0.87, respectively. Fixation indices ranged from ?0.41 to 0.44. Conclusions: These newly developed microsatellite markers for A. aurantiaca will be useful for future population genetic studies, specifically to detect the possible loss of genetic diversity due to habitat fragmentation.
机译：研究前提：为了评估森林破碎对多年生热带和观赏草多年生植物Aphhelandra aurantiaca（Acanthaceae）的遗传变异和种群结构的影响，我们开发了该物种的首个微卫星引物。方法和结果：从A. aurantiaca基因组文库中分离并表征了十四种微卫星标记，这些基因库富含二，三和四核苷酸重复基序。在来自四个自然种群的107个个体中评估了多态性。 14个遗传标记中有12个是多态性的。每个基因座的等位基因数量范围为2至12，观察到的和预期的杂合度分别为0.22至0.96和0.20至0.87。固色指数范围为约0.41至0.44。结论：这些新开发的用于A. aurantiaca的微卫星标记将对未来的人口遗传研究有用，特别是检测由于栖息地破碎而造成的遗传多样性丧失。

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