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Shape outline extraction software (DiaOutline) for elliptic Fourier analysis application in morphometric studies

机译:椭圆轮廓分析中的形状轮廓提取软件(DiaOutline)在形态计量学中的应用

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Premise of the Study Studies of plant cell and organ outline using shape analysis for taxonomic and morphological research have increased in the past decade. However, there are a limited number of available modern, intuitive, and easy software tools to conduct this work. Methods We developed a tool for shape outline extraction using MATLAB accompanied with R scripts to perform elliptic Fourier analysis. To demonstrate the shape tool, we applied the software and scripts for genera and species shape determinations of diatom (single cell) species with x‐, y‐, and x‐ + y‐shape symmetries. Results Using the shape analysis tool, we were able to identify and distinguish different diatom taxa based on forms representing size diminutions associated with population changes. Discussion Independent of symmetry, species were successfully distinguished using supervised and unsupervised analyses. We hope that these shape analysis tools will be used to add another metric to plant science studies.
机译:研究的前提在过去的十年中,使用形状分析进行分类和形态学研究的植物细胞和器官轮廓的研究有所增加。但是,仅有有限的可用现代,直观和简单的软件工具来执行此工作。方法我们开发了一种工具,该工具使用MATLAB和R脚本来进行椭圆轮廓提取,以进行形状轮廓提取。为了演示形状工具,我们将软件和脚本应用到具有x,y和x + y对称性的硅藻(单细胞)物种的属和物种形状确定中。结果使用形状分析工具,我们能够根据代表与种群变化相关的尺寸减小的形式,识别和区分不同的硅藻类群。讨论独立于对称性,使用监督和非监督分析成功区分了物种。我们希望这些形状分析工具将用于在植物科学研究中添加另一个指标。

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