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首页> 外文期刊>Advancements in Life Sciences >Molecular Characterization of EGLN1 Gene in Fast and Slow Moving Animals of Diverse Terrain
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Molecular Characterization of EGLN1 Gene in Fast and Slow Moving Animals of Diverse Terrain

机译：地形快慢动物中EGLN1基因的分子特征

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Background: Process of animal migration from their habitat to a new environment is alwaysproblematic due to low adaptive tendency, which ultimately affect their production, behavior andoverall performance. Current study is focused to explore the sequence diversity of EGLN1 gene inrelation to animal acclimatization at higher altitude under deprived oxygen or ability of betterutilization of oxygen which is considered to be liable for their agility in diverse terrains. Fast and slowmoving animals of plain and hilly terrains are potential species to study this phenomenon.Methods: Molecular characterization of EGLN1 gene was performed in sheep, goat, buffalo and camels ofPakistan as slow moving candidate species, while tiger, leopard, wolf, ibex, urial and markhor as fastmoving candidate species of diverse terrains by extraction their DNA from whole blood, followed by PCRamplification and sequence analysis of EGLN1 gene through BioEdit software. Later on, certainbioinformatics tools like MEGA, protparam and blast2sequence are used for the characterization of thenormal and mutant EGLN1 protein.Results: Current study revealed that goat and camel showed a nucleotide change at c.810 position. Whileall fast moving animals of higher altitude showed a nucleotide change at position c.406 except one sampleof markhor.Conclusion: Current study will assist to have an idea of sequence diversity of aforementioned candidategene responsible for adaption of animals in oxygen deprived environment of diverse terrain and may beresponsible for their agile behaviour of movement.
机译：背景：由于适应性趋势低，动物从栖息地迁移到新环境的过程始终存在问题，最终会影响动物的生产，行为和总体表现。当前研究的重点是探索在缺乏氧气或更好利用氧气的能力下，EGLN1基因与更高海拔的动物适应有关的序列多样性，这被认为是它们在不同地形中的敏捷性所致。方法：平原和丘陵地带的快慢动物是研究这一现象的潜在物种。方法：EGLN1基因的分子表征是在巴基斯坦的绵羊，山羊，水牛和骆驼中作为缓慢移动的候选物种，而老虎，豹，狼，高地山羊，从全血中提取其DNA，然后通过BioEdit软件对EGLN1基因进行PCR扩增和序列分析，从而将尿液和樟脑作为在各种地形中快速移动的候选物种。后来，某些生物信息学工具如MEGA，protparam和blast2sequence被用于表征正常和突变的EGLN1蛋白。虽然所有高空快速移动的动物除一个樟脑样品外都在c.406处显示核苷酸变化。结论：本研究将有助于人们理解上述候选基因的序列多样性，该基因负责动物在缺氧环境中的适应。可能是因为他们的动作敏捷。

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