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首页> 外文期刊>Advancements in Life Sciences >In vitro biochemical evaluation of methanol extract of Moringa oleifera pods on rat liver mitochondrial membrane permeability transition pore and lipid peroxidation
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In vitro biochemical evaluation of methanol extract of Moringa oleifera pods on rat liver mitochondrial membrane permeability transition pore and lipid peroxidation

机译：辣木荚甲醇提取物对大鼠肝线粒体膜通透性转换孔和脂质过氧化的体外生化评价

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Background: Moringa oleifera is a well-known world herbal plant for its amazing medicinal andnutritional properties. The effect of methanol extract of M. oleifera can be useful in managingdiseases associated with mitochondrial membrane permeability transition pore and lipidperoxidation.Methods: Evaluation was done at varying concentrations of the methanol pods extract on mitochondrialmembrane permeability transition pore opening (swelling) and Fe2+- H2O2-EDTA (Fenton reaction)-induced lipid peroxidation in vitro. Five male albino rats (weighed 120-250 g) were anaesthetized andsacrificed; the liver was excised and homogenized to obtain mitochondria pellets. This study analyzed theeffect of varying concentrations of methanol pods extract of M. oleifera at 50, 150, and, 300 μg/mlrespectively. The effects of M. oleifera varying concentration in vitro was determined usingmalondialdehyde reaction quantified at 532 nm in a UV- spectrophotometer as index for lipidperoxidation and spectrophotometric absorptions at 520 nm was observed as an index of mitochondrialmembrane permeability pore respectively.Results: Varying concentrations of methanol pods extract of M. oleifera at 50, 150, and 300 μg/ml in thepresence and absence of triggering agent (Ca2+) inhibited opening of mitochondria membranepermeability transition pore while 0.25, 0.50 and 1.00 mg/ml inhibited lipid peroxidation inducedmitochondria of the rat liver respectively in a concentration dependent mode.Conclusion: The results suggest that methanol extract of M. oleifera pods at high concentrations (such as300 μg/ml and 1.00 mg/ml respectively) may inhibit mitochondrial membrane permeability transitionpore opening and lipid peroxidation.
机译：背景：辣木是世界著名的草药植物，以其惊人的药用和营养特性而闻名。油茶甲醇提取物的作用可用于处理与线粒体膜通透性转变孔和脂质过氧化有关的疾病。方法：在不同浓度的甲醇豆荚提取物上，评估线粒体膜通透性转变孔的开度（膨胀）和Fe2 + -H2O2。 -EDTA（芬顿反应）诱导体外脂质过氧化。麻醉并处死5只雄性白化病雄性大鼠（体重120-250 g）;切除肝脏并匀浆以获得线粒体沉淀。这项研究分析了浓度分别为50、150和300μg/ ml的油茶分枝杆菌甲醇荚提取物的影响。使用紫外分光光度计在532 nm处定量的丙二醛反应作为脂质过氧化的指标，并在520 nm处以分光光度法吸收作为线粒体膜通透性孔隙的指标，来测定油茶分枝杆菌在体外的影响。结果：甲醇浓度变化在存在和不存在触发剂（Ca2 +）的情况下，油果毛豆提取物浓度分别为50、150和300μg/ ml时可抑制线粒体膜通透性过渡孔的开放，而0.25、0.50和1.00 mg / ml则可抑制脂质过氧化诱导的大鼠肝线粒体结论：结果表明，高浓度油茶荚果甲醇提取物（分别为300μg/ ml和1.00 mg / ml）可能抑制线粒体膜通透性过渡孔的开放和脂质过氧化。

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