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首页> 外文期刊>Archives of Biological Sciences >EFFICIENT IN VITRO PROPAGATION FROM PRECONDITIONED EMBRYONIC AXES OF TURKISH COWPEA (VIGNA UNGUICULATA L.) CULTIVAR AKKIZ
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EFFICIENT IN VITRO PROPAGATION FROM PRECONDITIONED EMBRYONIC AXES OF TURKISH COWPEA (VIGNA UNGUICULATA L.) CULTIVAR AKKIZ

机译：土耳其CWPEA（VIGNA UNGUICULATA L.）早熟胚核的早熟胚轴的体外繁殖效率高

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摘要

Cowpea is an important grain legume crop. The study reports an efficient in vitro multiplication and shoots regeneration protocol from preconditioned embryonic axes of the Turkish cowpea cultivar Akkiz. The embryonic axes were preconditioned with 10 mg/l BA on agar solidified MS medium for 5 days. Thereafter they were cultured on MS medium containing 0.25, 0.50, 0.75 and 1.00 mg/l BA with or without 0.10 mg/l NAA. Mean frequency (%) of shoot regeneration, number of shoots per explant and shoot length decreased with each increase in BA concentration used singly. However, a positive increase was recorded in all parameters in the presence of 0.10 mg/l NAA in the regeneration medium. A maximum mean number of 10.33 shoots per explant was recorded on an MS medium containing 1.00 mg/l BA -0.1 mg/l NAA. Regenerated shoots were rooted on an MS medium containing 0.50 mg/l IBA. Rooted plants were acclimatized at room temperature in soil mix contained in pots where they were subjected to an intermittent mistwater spray for 24 h that maintained 90% relative humidity during the first few days which was gradually reduced to 40% for 10 days. All plants flowered and set seeds in a greenhouse after 3 months.
机译：豆是一种重要的谷物豆类作物。这项研究报告了从土耳其pea豆品种Akkiz的预处理胚轴进行的高效体外繁殖和芽再生方案。胚轴在琼脂固化的MS培养基上用10 mg / l BA预处理5天。之后，将它们在含有0.25、0.50、0.75和1.00mg / l BA的MS培养基上培养，所述培养基具有或不具有0.10mg / l NAA。芽的平均发生频率（％），每株外植体的芽数和茎长随单独使用BA浓度的增加而降低。但是，在再生培养基中存在0.10 mg / l NAA的情况下，所有参数均记录为正增加。在含有1.00 mg / l BA -0.1 mg / l NAA的MS培养基上记录了每个外植体的最大平均芽数为10.33。再生的芽生根于含有0.50 mg / l IBA的MS培养基上。将已生根的植物在室温下适应盆栽中所含的土壤混合物，在其中进行间歇性雾水喷雾24小时，该喷雾在开始的几天内保持90％的相对湿度，然后逐渐降低至40％，持续10天。 3个月后，所有植物开花并结实于温室中。

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