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首页> 外文期刊>Arab Journal of Urology >Down-regulated claudin-7 immunoexpression in urothelial carcinoma of the urinary bladder
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Down-regulated claudin-7 immunoexpression in urothelial carcinoma of the urinary bladder

机译：膀胱尿路上皮癌中claudin-7免疫表达下调

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Objectives To analyse the gene-expression level of claudin-7 in urothelial carcinoma (UC) of the urinary bladder, and its relationship with clinicopathological variables. Materials and methods This study included 68 specimens of UC of the bladder, comprising 35 with non-muscle-invasive (NMI), stage Ta–T1, and 33 with muscle-invasive (MI) tumours, T2–T4, and 26 of normal urothelium (NU). Total RNA was extracted and 1 μg was reverse transcribed using a cDNA kit. RT-PCR was conducted using SYBR Green I dye to examine the expression levels of the target gene (claudin-7) and the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase. Using confocal-laser scanning light microscopy, immunohistochemistry (IHC) was used to validate the RT-PCR data. The correlation between claudin-7 and the clinicopathological variables was assessed. Results Claudin-7 was down-regulated in UC samples compared to NU samples ( P 0.001). NMI (Ta–T1) tumours had significantly higher claudin-7 expression than MI (?pT2) tumours ( P = 0.012). There was no significant difference between patients with G1-2 tumours and those with G3 tumours ( P = 0.19). There was no significant difference between patients with recurrent NMI UC and those with no recurrence ( P = 0.61). IHC showed a lower expression of claudin-7 in the UC samples than NU samples, and in MI UC than in NMI UC. Conclusions These results indicate that a reduced expression of claudin-7 correlates with the invasiveness and progression of UC of the urinary bladder. Further studies are needed to validate claudin-7 as a marker for UC.
机译：目的分析claudin-7在膀胱尿路上皮癌（UC）中的基因表达水平及其与临床病理变量的关系。材料和方法这项研究包括68例膀胱UC样本，其中35例具有非肌肉侵袭性（NMI），Ta-T1期，33例具有肌肉侵袭性（MI）肿瘤，T2-T4，以及26例正常尿路上皮（NU）。提取总RNA，并使用cDNA试剂盒反转录1μg。使用SYBR Green I染料进行RT-PCR，以检查靶基因（claudin-7）和管家基因甘油醛-3-磷酸脱氢酶的表达水平。使用共聚焦激光扫描光镜，免疫组织化学（IHC）用于验证RT-PCR数据。评估claudin-7与临床病理变量之间的相关性。结果与NU样品相比，UC样品中Claudin-7的表达下调（P <0.001）。 NMI（Ta–T1）肿瘤的claudin-7表达明显高于MI（ΔpT2）肿瘤（P = 0.012）。 G1-2肿瘤患者和G3肿瘤患者之间无显着差异（P = 0.19）。复发性NMI UC患者与未复发的患者之间无显着差异（P = 0.61）。 IHC在UC样品中显示出claudin-7的表达低于NU样品，在MI UC中显示出比NMI UC低。结论这些结果表明claudin-7表达的降低与膀胱UC的侵袭性和进展有关。需要进一步的研究以验证claudin-7作为UC的标志物。

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