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首页> 外文期刊>Archivos Latinoamericanos de Produccion Animal >Effects of addition of a liquid supplement supplying lactic acid bacteria to diets of alfalfa-concentrate and corn stover-concentrate in sheep
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Effects of addition of a liquid supplement supplying lactic acid bacteria to diets of alfalfa-concentrate and corn stover-concentrate in sheep

机译：在绵羊苜蓿浓缩液和玉米秸秆浓缩液中添加补充乳酸菌的液体补充剂的效果

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To determine the effects of a liquid supplement containing lactic acid bacteria(SULIBAL) on rumen fermentation and utilization of mixed diets of alfalfa or corn stover and commercial concentrates, four adult male sheep (52 ± 10kg), fitted with rumen canulae, were fed four treatments composed of (%): T1 (alfalfa 55 and concentrate 45), T2 (alfalfa 50, concentrate 40 and SULIBAL 10), T3 (stover 55 and concentrate 45), T4 (stover 50, concentrate 40 and SULIBAL 10), with T1 and T3 serving as control to T2 and T4, respectively. The liquid supplement was formulated with diverse ingredients, most importantly fresh whey from cheese making and yogurt as sources of lactic acid bacteria. Rumen fermentation was characterized by measurement of pH, levels of NH3 and VFA, and kinetics of in situ DM disappearance; N balance in the body was also determined. Mean DM consumption (g/d) in T1 thru T4 was: 1148, 1291, 887, and 1023, with positive effects (P 3/L, ammonia concentration in rumen liquid climbed most rapidly post-prandially in T2 and T4, reached maxima (93 to 112 mg/L) at 4 h and thereafter decreased progressively until, at 12 h, reaching approximately initial levels in T1 and T2 and values below 30 mg/L in T2 and T4. Rumen concentration of total VFA showed principally an advantage of alfalfa over stover, while the molar percentage of propionic acid responded positively to the liquid supplement when this was combined with stover (T4). in situ DM degradation at 96 h of incubation surpassed 60% in all treatments, while estimated true DM digestibility favored the addition of SULIBAL (T2, 83.68; T4, 76.78=) over the controls (T1, 71.95; T3, 73.01). Nitrogen digestibility and retention were markedly higher in T1 and T2 (alfalfa) than T3 and T4 (stover), while between the latter two the presence of SULIBAL gave some improvement. It is concluded that addition of the liquid supplement to these diets proved to be beneficial, by tending to improve in vivo consumption and in situ estimated digestibility and possibly promoting the synthesis of microbial protein in the rumen (judging from the indirect evidence of formation and disappearance of NH3 ).
机译：为了确定含乳酸菌的液体补充剂（SULIBAL）对瘤胃发酵以及苜蓿或玉米秸秆与市售浓缩饲料混合日粮的利用的影响，给四只装有瘤胃插管的成年雄性绵羊（52±10kg）喂了四只由以下各项组成的处理（％）：T1（苜蓿55和精矿45），T2（苜蓿50，精矿40和SULIBAL 10），T3（苜蓿55和精矿45），T4（苜蓿50，精矿40和SULIBAL 10）， T1和T3分别作为对T2和T4的控制。液体补充剂由多种成分配制而成，最重要的是来自奶酪制造和酸奶的新鲜乳清，它们是乳酸菌的来源。通过测量pH，NH 3 和VFA的含量以及原位DM消失的动力学来表征瘤胃发酵。还确定了体内的氮平衡。 T1至T4的平均DM消费量（g / d）为：1148、1291、887和1023，具有积极的作用（P 3 / L，瘤胃液中的氨浓度在T2餐后上升最快。 T4在4 h达到最大值（93至112 mg / L），然后逐渐下降，直到12 h，在T1和T2达到大约初始水平，在T2和T4达到30 mg / L以下。研究表明，紫花苜蓿的主要优势是比苜蓿更具优势，而丙酸的摩尔百分数与秸秆（T4）结合时对液体补充剂呈正响应;在所有处理中，孵育96小时的原位DM降解率均超过60％，真正的DM消化率有利于添加SULIBAL（T2，83.68; T4，76.78 =），而不是对照（T1，71.95; T3，73.01）.T1和T2（苜蓿）中的氮消化率和截留率显着高于T3和T4（秸秆），而在后两者之间，SULIBAL的存在有所改善。结论是，向这些饮食中添加液体补充剂被证明是有益的，因为它倾向于改善体内消耗和原位估计的消化率，并可能促进瘤胃中微生物蛋白质的合成（从间接证据表明，这种食物的形成和消失）。 NH 3 ）。

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