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首页> 外文期刊>Artificial cells, blood substitutes, and immobilization biotechnology. >Construction of a Potentiometric Glutamate Biosensor for Determination of Glutamate in Some Real Samples
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Construction of a Potentiometric Glutamate Biosensor for Determination of Glutamate in Some Real Samples

机译：用于测定某些实际样品中谷氨酸的电位型谷氨酸生物传感器的构建

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: The potentiometric glutamate biosensor based on ammonium-selective poly(vinylchloride) (PVC) membrane electrode was constructed by chemically immobilizing glutamate oxidase. Ammonium ions produced after an enzymatic reaction were determined potentiometrically. We determined the optimum working conditions of the biosensor such as buffer concentration, buffer pH, lifetime, response time, linear working range, kinetic constants (Km and Vmax) of glutamate oxidase enzyme used for biosensor construction values, and other response characteristics. Additionally, glutamate assay in some real samples such as chicken bullion, healthy human serum, and commercial multipower amino acid mixture were also successfully carried out. The results showed good agreement with previously reported values.
机译：：通过化学固定谷氨酸氧化酶，构建了基于铵选择性聚氯乙烯（PVC）膜电极的电位型谷氨酸生物传感器。酶促反应后产生的铵离子用电位法测定。我们确定了生物传感器的最佳工作条件，例如缓冲液浓度，缓冲液pH，寿命，响应时间，线性工作范围，用于生物传感器构建值的谷氨酸氧化酶的动力学常数（Km和Vmax）以及其他响应特性。此外，还成功地在一些真实样品中进行了谷氨酸测定，例如金条，健康的人血清和市售多倍氨基酸混合物。结果表明与先前报道的值吻合良好。

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