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首页> 外文期刊>Artificial cells, blood substitutes, and immobilization biotechnology. >Photochemical Immobilization of Cucurbita Fruit Ascorbate Oxidase onto Polyethylene Disc for Determination of Ascorbate in Serum and Foodstuffs
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Photochemical Immobilization of Cucurbita Fruit Ascorbate Oxidase onto Polyethylene Disc for Determination of Ascorbate in Serum and Foodstuffs

机译：将南瓜属水果抗坏血酸氧化酶光化学固定在聚乙烯膜片上测定血清和食品中的抗坏血酸

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: An ascorbate oxidase purified from the green fruit of zucchini squash (Cucurbita pepo medullosa) was immobilized photochemically on a polyethylene disc with 85% retention of initial activity of free enzyme. The optimum pH (5.5) was unchanged, while Km was decreased. The polyethylene discs were employed for determination of ascorbic acid in serum and foodstuffs. The working linear range was 2.8 μM to 16 μM. The mean value of ascorbic acid in serum as measured by the method was 0.16 mg/dl in males and 0.209 mg/dl in females. The immobilized enzyme was used 100 times over 4 months, when stored at 4°C.
机译：：从南瓜西葫芦（西葫芦）的绿色果实中纯化的抗坏血酸氧化酶光化学固定在聚乙烯圆盘上，保留了85％的游离酶初始活性。最佳pH（5.5）不变，而Km降低。聚乙烯圆盘用于测定血清和食品中的抗坏血酸。工作线性范围为2.8μM至16μM。通过该方法测得的血清中抗坏血酸的平均值在男性中为0.16 mg / dl，在女性中为0.209 mg / dl。当在4°C下保存时，固定化酶在4个月内使用100次。

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