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首页> 外文期刊>Artificial cells, blood substitutes, and immobilization biotechnology. >Potential c-myc Antisense Oligonucleotide Carriers: PCl/PEG/PEI and PLL/PEG/PEI
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Potential c-myc Antisense Oligonucleotide Carriers: PCl/PEG/PEI and PLL/PEG/PEI

机译：潜在的c-myc反义寡核苷酸载体：PC1 / PEG / PEI和PLL / PEG / PEI

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: In this work, positively charged, micelle-forming polymers were synthesized and used as a model vector to deliver antisense oligodeoxynucleotide (ASODN) into melanoma cells. Polymers and polymer/ASODN complexes were characterized by DLS according to size, charge, and critical micelle concentration. Nanosize and spherical complexes were observed by AFM. Complexes did not reveal significant toxicity to melanoma cells. Antiproliferative effect of the complexes was observed by immunocytochemical staining and estimated as 56.8% with N/P:9. High amount of apoptosis and very small amount of necrosis were estimated. According to the results, these positively charged polymers forming micelle-like structures seem promising as ASODN carriers.
机译：：在这项工作中，合成了带正电荷，形成胶束的聚合物，并将其用作模型载体，以将反义寡聚脱氧核苷酸（ASODN）输送到黑素瘤细胞中。根据大小，电荷和临界胶束浓度，通过DLS表征聚合物和聚合物/ ASODN复合物。通过AFM观察到纳米尺寸和球形复合物。复合物未显示出对黑素瘤细胞的显着毒性。通过免疫细胞化学染色观察到复合物的抗增殖作用，用N / P：9估计为56.8％。估计细胞凋亡高且坏死很少。根据结果​​，这些形成胶束状结构的带正电的聚合物有望用作ASODN载体。

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