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首页> 外文期刊>Australian Journal of Crop Science >In vitro micropropagation through cotyledonary node culture of castor bean (Ricinus communis L.)
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In vitro micropropagation through cotyledonary node culture of castor bean (Ricinus communis L.)

机译：蓖麻子叶子叶节培养的体外微繁殖

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摘要

An efficient plant regeneration protocol was described for castor (Ricinus communis L.) using whole cotyledonary nodes as explant. Seeds were surface sterilized with 0.1% (w/v) mercuric chloride and germinated in growth regulator- free MS medium. Cotyledonary nodes were excised from 5-7 days old seedlings and were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP, Kin singly or in combination with NAA. Use of BAP at 3.0 mg.l-1 induced the highest frequency (85%) of shoot induction as well as maximum number of shoots per explant (12.56). Proliferated shoot clusters were elongated in 1.0 mg.l-1 BAP in combination with 0.25 mg.l-1 GA3 . For root induction, in vitro shoots were transferred to rooting media containing NAA or IBA. The highest rooting frequency (87.5%) as well as highest number of roots (10.5) was observed in MS medium supplemented with 1.0 mg.l-1 NAA. Regenerated plantlets were acclimatized successfully in the growth room for further development.
机译：描述了一种使用全子叶节作为外植体的蓖麻（Ricinus communis L.）的有效植物再生方案。种子用0.1％（w / v）氯化汞进行表面灭菌，并在无生长调节剂的MS培养基中发芽。从5-7天大的幼苗中切下子叶节，并在Murashige和Skoog（MS）培养基上培养，Murashige和Skoog（MS）培养基单独添加或与NAA组合添加不同浓度的BAP。使用3.0 mg.l-1的BAP可以诱导最高的芽诱导频率（85％），以及每个外植体的最大芽数（12.56）。在与0.25 mg.l-1 GA3组合的1.0 mg.l-1 BAP中将增殖的芽簇拉长。为了进行根诱导，将体外芽转移到含有NAA或IBA的生根培养基中。在补充有1.0 mg.1-1 NAA的MS培养基中观察到最高的生根频率（87.5％）和最高的根数（10.5）。再生苗已成功地在生长室中适应了进一步的发展。

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