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首页> 外文期刊>Australian Journal of Crop Science >Electrical conductivity test for evaluating physiological quality in snap bean (Phaseolus vulgaris L.) seeds
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Electrical conductivity test for evaluating physiological quality in snap bean (Phaseolus vulgaris L.) seeds

机译：电导率测试用于评估四季豆种子的生理品质

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摘要

Snap bean is a variety of common bean (Phaseolus vulgaris L.) that is cultivated and consumed as a vegetable worldwide. In orderto optimize the crop’s production, germination tests are used to ensure that seeds are high in quality. However, because thesetests possess inherent limitations, vigor tests, such as the electrical conductivity test, may be used instead to assess seed quality.The goal of the present study was to develop a standardized methodology for measuring electrical conductivity in snap bean seedthat could reliably assess the physiological quality of seed batches. Groups of seeds (n = 50) from each of seven snap beangenotypes (UEL 1, UEL 2, HAV 21, HAV 22, HAV 34, HAV 35, and HAV 42) were immersed in distilled water (75 or 150 mL) forvarious immersion periods (2, 4, 8, 12, 16, 20, or 24 h), using a completely randomized 7 × 2 × 7 factorial design, with eightreplicates per treatment. After immersion, the electrical conductivity of seeds from each treatment group was measured in μS cm -1using a conductivity meter. The group means were subjected to ANOVA and the Scott-Knott test (p ≤ 0.05). The optimal conditionsfor the electrical conductivity test in snap bean seeds were a 16-h immersion in 75 mL of water. Under these conditions, genotypescould be clearly differentiated based on their seed vigor, optimizing the measurement of seed quality.
机译：四季豆是在世界范围内作为蔬菜种植和消费的多种普通豆（菜豆）。为了优化农作物的产量，使用了发芽测试来确保种子的高质量。但是，由于这些测试具有固有的局限性，因此可以使用活力测试（例如电导率测试）来替代评估种子质量。本研究的目的是开发一种标准方法来测量菜豆种子的电导率，从而可以可靠地评估种子批次的生理品质。将七种速生甜菜型（UEL 1，UEL 2，HAV 21，HAV 22，HAV 34，HAV 35和HAV 42）中的每一种种子组（n = 50）浸入蒸馏水（75或150 mL）中进行各种浸没周期（2、4、8、12、16、20或24小时），采用完全随机的7×2×7因子设计，每次治疗重复八次。浸没后，使用电导率仪以μScm -1测量每个处理组的种子的电导率。对组平均值进行ANOVA和Scott-Knott检验（p≤0.05）。在四季豆种子中进行电导率测试的最佳条件是在75 mL水中浸泡16小时。在这些条件下，应根据种子的活力明确区分基因型，从而优化种子质量的测量。

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