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首页> 外文期刊>Autoimmune diseases >A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus
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A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus

机译：一种实时，连续，基于荧光的系统性红斑狼疮抗DNA抗体活性分析的新方法

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Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by the production of antibodies against a variety of self-antigens including nucleic acids. These antibodies are cytotoxic, catalytic (hydrolyzing DNA, RNA, and protein), and nephritogenic. Current methods for investigating catalytic activities of natural abzymes produced by individuals suffering from autoimmunity are mostly discontinuous and often employ hazardous reagents. Here we demonstrate the utility of dual-labeled, fluorogenic DNA hydrolysis probes in highly specific, sensitive, continuous, fluorescence-based measurement of DNA hydrolytic activity of anti-ssDNA abzymes purified from the serum of patients suffering from SLE. An assay for the presence and levels of antibodies exhibiting hydrolytic activity could facilitate disease diagnosis, prediction of flares, monitoring of disease state, and response to therapy. The assay may allow indirect identification of additional targets of anti-DNA antibodies and the discovery of molecules that inhibit their activity. Combined, these approaches may provide new insights into molecular mechanisms of lupus pathogenesis.
机译：系统性红斑狼疮（SLE）是一种自身免疫性疾病，其特征是产生针对多种自身抗原（包括核酸）的抗体。这些抗体具有细胞毒性，催化作用（水解DNA，RNA和蛋白质）并具有生肾作用。研究患有自身免疫的个体产生的天然抗体的催化活性的当前方法大部分是不连续的，并且经常使用危险试剂。在这里，我们证明了双标记的荧光DNA水解探针在高度特异性，灵敏，连续，基于荧光的从SLE患者血清中纯化的抗SSDNA酶的DNA水解活性的测量中的实用性。表现出水解活性的抗体的存在和水平的测定可以促进疾病的诊断，耀斑的预测，疾病状态的监测以及对治疗的反应。该测定法可以间接鉴定抗DNA抗体的其他靶标并发现抑制其活性的分子。结合起来，这些方法可能为狼疮发病机理的分子机制提供新的见解。

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