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首页> 外文期刊>Ibnosina Journal of Medicine and Biomedical Sciences >Neurohistochemical studies of adolescent rats’ prefrontal cortex exposed to prenatal nicotine
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Neurohistochemical studies of adolescent rats’ prefrontal cortex exposed to prenatal nicotine

机译：暴露于产前尼古丁的青春期大鼠前额叶皮层的神经组织化学研究

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Twenty-four mature female Wistar rats were mated and grouped according to Trimester into Control and Treated groups. Nicotine was administered intra-peritoneally to pregnant Wistar rats in the treated groups, while normal saline was given to the control groups, at each of the three Trimesters. The animals were allowed to litter and the pups were allowed to grow till postnatal day 35, when they were sacrificed and the brain removed and weighed. The prefrontal cortex was excised and either fixed in 4% paraformaldehyde for tissue histology or homogenised in sucrose solution for enzyme studies (alkaline phosphatase, lactate dehydrogenase and glucose-6-phosphate dehydrogenase). Enzyme studies showed derangement in biochemical status of the prefrontal cortex of all the nicotine-exposed animals compared with their respective controls, and corresponding morphological and histological alterations, especially in animals exposed to nicotine during their 2 nd and 3 rd weeks of foetal life. The morphohistological and biochemical derangements that occur during neurodevelopment of nicotine-exposed offspring persist into adolescent life, and could underlie the neurological dysfunctions associated with such individuals. Keywords: adolescents, prenatal nicotine, prefrontal cortex, enzymes, histology
机译：将24只成年雌性Wistar大鼠交配并根据Trimester分为对照组和治疗组。在三个三个月中的每个三个月中，在治疗组中对怀孕的Wistar大鼠腹膜内给予尼古丁，而对照组给予生理盐水。允许动物乱扔垃圾，让幼犬生长直到出生后第35天，然后处死它们，取出大脑并称重。切除前额叶皮层并固定在4％多聚甲醛中以进行组织组织学检查，或在蔗糖溶液中匀浆以进行酶研究（碱性磷酸酶，乳酸脱氢酶和6磷酸葡萄糖脱氢酶）。酶研究显示，所有暴露于尼古丁的动物与其各自的对照组相比，前额叶皮层的生化状态发生了紊乱，并发生了相应的形态学和组织学改变，尤其是在胎儿生命的第二周和第三周暴露于尼古丁的动物中。在暴露于尼古丁的后代的神经发育过程中发生的形态组织学和生化异常一直持续到青春期，并且可能是与此类个体相关的神经功能障碍的基础。关键词：青少年，产前尼古丁，前额叶皮层，酶，组织学

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