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首页> 外文期刊>Indian Journal of Medical Microbiology >Identification of plasmid-mediated quinolone resistance genes qnrA1, qnrB1 and aac(6')-1b-cr in a multiple drug-resistant isolate of Klebsiella pneumoniae from Chennai
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Identification of plasmid-mediated quinolone resistance genes qnrA1, qnrB1 and aac(6')-1b-cr in a multiple drug-resistant isolate of Klebsiella pneumoniae from Chennai

机译：钦奈肺炎克雷伯菌多重耐药株中质粒介导的喹诺酮耐药基因qnrA1，qnrB1和aac（6'）-1b-cr的鉴定

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Purpose: Resistance to fluoroquinolones, a commonly prescribed antimicrobial for Gram-negative and Gram-positive microorganisms, is of importance in therapy. The purpose of this study was to screen for the presence of Plasmid-Mediated Quinolone Resistance (PMQR) determinants in clinical isolates of Klebsiella pneumoniae. Materials and Methods: Extended-Spectrum Beta-Lactamase (ESBL) isolates of K. pneumoniae collected during October 2009 were screened by the antimicrobial susceptibility test. The plasmids from these isolates were analysed by specific Polymerase chain Reaction (PCR) for qnrA, qnrB and aac(6')-1b. The amplified products were sequenced to confirm the allele. Results: Our analysis showed that 61% out of the 23 ESBL K. pneumoniae isolates were resistant to ciprofloxacin and 56% to levofloxacin. The PMQR was demonstrated by transforming the plasmids from two isolates P12 and P13 into E. coli JM109. The PMQR gene qnrA was found in 16 isolates and qnrB in 11 isolates. The plasmid pKNMGR13 which conferred an minimum inhibitory concentration (MIC) of more than 240 ΅g/ml in sensitive E. coli was found to harbour the qnrA1 and qnrB1 allele. Furthermore, the gene aac(6')-1b-cr encoding a variant aminoglycoside 6'-N Acetyl transferase which confers resistance to fluoroquinolones was found in the same plasmid. Conclusions: Our report shows the prevalence of PMQR mediated by qnrA and qnrB in multidrug-resistant K. pneumoniae isolates from Chennai. A multidrug-resistant plasmid conferring high resistance to ciprofloxacin was found to harbour another PMQR gene, aac(6')-1b-cr mutant gene. This is the first report screening for PMQR in K. pneumoniae isolates from India.
机译：目的：对氟喹诺酮类药物（一种对革兰氏阴性和革兰氏阳性微生物的常用处方抗菌药物）的耐药性在治疗中很重要。这项研究的目的是筛选肺炎克雷伯菌的临床分离株中质粒介导的喹诺酮抗性（PMQR）决定簇的存在。材料和方法：通过抗菌药敏试验筛选了2009年10月收集的肺炎克雷伯菌的广谱β-内酰胺酶（ESBL）分离株。通过针对qnrA，qnrB和aac（6'）-1b的特异性聚合酶链反应（PCR）分析了这些分离物中的质粒。对扩增产物进行测序以确认等位基因。结果：我们的分析表明，在23株ESBL肺炎克雷伯菌中，有61％对环丙沙星耐药，对左氧氟沙星有56％耐药。通过将来自两个分离株P12和P13的质粒转化到大肠杆菌JM109中，证明了PMQR。在16个菌株中发现了PMQR基因qnrA，在11个菌株中发现了qnrB。发现质粒pKNMGR13在敏感大肠杆菌中的最小抑菌浓度（MIC）超过240μg/ ml，被发现带有qnrA1和qnrB1等位基因。此外，在同一质粒中发现了编码aac（6'）-1b-cr基因aac（6'）-1b-cr，该变体具有赋予对氟喹诺酮类药物抗性的氨基糖苷6'-N乙酰基转移酶。结论：我们的报告显示了由qnrA和qnrB介导的PMQR在金奈州多药耐药肺炎克雷伯菌分离物中的流行。发现对环丙沙星具有高度耐药性的多药耐药质粒带有另一个PMQR基因aac（6'）-1b-cr突变基因。这是首次对印度肺炎克雷伯菌中PMQR的筛查报告。

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