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首页> 外文期刊>Indonesian Journal of Forestry Research >BIOLOGICAL ACTIVITIES AFFORDED BY THE EXTRACT FROM RARU BARK TO INHIBIT ACTION OF ALPHA- GLUCOSIDASE ENZYMES
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BIOLOGICAL ACTIVITIES AFFORDED BY THE EXTRACT FROM RARU BARK TO INHIBIT ACTION OF ALPHA- GLUCOSIDASE ENZYMES

机译：罗拉皮提取物对α-葡糖苷酶酶的抑制作用所具有的生物活性

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Raru ( S h o r e a b a l a no ca r poi d e s Sym) signifies one of the tree species that grows widespread in Sumatra Island. Its bark portion is commonly used by local villagers as additional ingredient mixed to nira (sugar palm juice). This addition is intended to make the juice more durable and also to enrich its taste after the juice is previously fermented to become traditional toddy beverage or the so-call “tuak”. Local villagers believe that raru bark can reduce the level of blood sugar. As the relevance, the research was conducted to confirm that the extract from raru bark could afford its biological activities to inhibit alpha-glucosidase enzyme through its characterization, quantification, and isolation of its boactive compound. The extraction was performed using two methods (i.e.reflux and maceration techniques). Result revealed that the bark extract obtained from both techniques contained polyphenol compounds: flavonoid, saponin and tannin. Further, raru-bark extract from the reflux and maceration techniques could inhibit the action of alpha glucosidase enzymes on carbohydrate substrate ( i.e. p -nitrophenil-α-D-glucopyranose), at respectively 90.67% and 97.33%. Meanwhile, the inhibition activities afforded by the patented drug as a control (i.e. glucobay) equaled to 97.05%. Assesment using UV-VIS spectroscopy, showed that the maximum spectrum of bioactive compound in the extract was at the wave length of 288.6 nm. Scrutiny using FTIR spectroscopy could identif y the presence of aromatic groups in the compound, containing -OH, C-H, C=C, C-O and C-H bond types. Analysis using GC-MS exhibited that the compound had molecular weight of 390 with molecular structure as C20H22O8. Ultimately, data analysis scrutiny with the aid of NMR judged the most plausible compound as bioactive was 4-Glucosyl-3, 4’, 5-trihydroxystilbene.
机译：拉鲁（Shuer a ba l'a no ca r poi d s Sym）象征着在苏门答腊岛广泛分布的一种树种。它的树皮部分通常被当地村民用作混合尼拉（糖棕榈汁）的其他成分。这种添加旨在使果汁更耐用，并且在果汁经过预先发酵成为传统的托迪饮料或所谓的“ tuak”之后，还可以丰富其口味。当地村民认为，拉鲁树皮可以降低血糖水平。作为相关性，进行了研究以证实罗拉树皮提取物可以通过其活性化合物的表征，定量和分离来提供抑制α-葡萄糖苷酶的生物活性。提取使用两种方法（即回流和浸渍技术）进行。结果表明，从两种技术获得的树皮提取物均含有多酚类化合物：类黄酮，皂苷和单宁。此外，从回流和浸软技术获得的树皮提取物可以抑制α葡萄糖苷酶对碳水化合物底物（即对硝基苯菲-α-D-吡喃葡萄糖）的作用，分别为90.67％和97.33％。同时，该专利药物作为对照（即葡糖苷）提供的抑制活性等于97.05％。使用UV-VIS光谱进行的评估表明，提取物中生物活性化合物的最大光谱在288.6 nm的波长处。使用FTIR光谱进行仔细检查可以确定化合物中是否存在芳族基团，这些芳族基团具有-OH，C-H，C = C，C-O和C-H键类型。用GC-MS分析表明该化合物的分子量为390，分子结构为C 20 H 22 O 8。最终，借助NMR进行的数据分析审查认为，最有可能具有生物活性的化合物是4-葡萄糖基-3，4'，5-三羟基sti。

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