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首页> 外文期刊>Interdisciplinary perspectives on infectious diseases >Contamination of Hospital Water Supplies in Gilan, Iran, withLegionella pneumophila, Escherichia coli,andPseudomonas aeruginosa
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Contamination of Hospital Water Supplies in Gilan, Iran, withLegionella pneumophila, Escherichia coli,andPseudomonas aeruginosa

机译：伊朗吉兰医院的医院供水受到肺炎军团菌，大肠杆菌和铜绿假单胞菌的污染

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This study is designed to determine the contamination degree of hospital water supplies withPseudomonas aeruginosa, Legionella pneumophila, andE. coliin Gilan, Iran. Samples were collected directly into sterile containers and concentrated by centrifuge. Half part of any sample transferred to yeast extract broth and the second part transferred to Trypticase Soy Broth and incubated for 3 days. DNA was extracted by using commercial kit. Four rounds of PCR were performed as follows: multiplex PCR for detectingPseudomonas aeruginosa, Integron 1, and Metallo-β-lactamases gene; PCR for detectingLegionella pneumophilaandmipgene separately; PCR for detectingE. coli; and another PCR for detecting whole bacterial presence. Contamination rates of cold, warm, and incubator water samples withP. aeruginosa, were 16.6%, 37.5%, and 6.8% consequently. Degrees of contamination withL. pneumophilawere 3.3%, 9.3%, and 10.9% and withE. coliwere zero, 6.2%, and zero. Total bacterial contamination of cold, warm, and incubator water samples was 93.3%, 84.4%, and 89.0% consequently. Metallo-β-lactamases gene was found in 20.0% of all samples. Contamination degree withP. aeruginosawas considerable and withL. pneumophilawas moderate. Metallo-β-lactamases gene was found frequently indicating widespread multiple drug resistance bacteria. We suggest using new decontamination method based on nanotechnology.
机译：本研究旨在确定铜绿假单胞菌，嗜肺军团菌和大肠杆菌对医院供水的污染程度。大肠杆菌素吉兰（伊朗）。将样品直接收集到无菌容器中并通过离心浓缩。将任何样品的一半转移到酵母提取液中，第二部分转移到胰蛋白酶大豆肉汤中，孵育3天。通过使用商业试剂盒提取DNA。进行四轮PCR，如下：用于检测铜绿假单胞菌，整合子1和金属β-内酰胺酶基因的多重PCR; PCR分别检测嗜肺军团菌和双歧杆菌基因;用于检测E的PCR。大肠杆菌另一个用于检测整个细菌存在的PCR。 P对冷，热和培养箱中水样品的污染率。因此，铜绿假单胞菌分别为16.6％，37.5％和6.8％。 L的污染程度。肺炎球菌3.3％，9.3％和10.9％并伴有E. coli分别为零，6.2％和零。因此，冷水，温水和培养箱中水样的总细菌污染分别为93.3％，84.4％和89.0％。在所有样品中发现20.0％的金属β-内酰胺酶基因。污染度P。铜绿泽相当可观。肺炎中等。经常发现金属β-内酰胺酶基因，表明广泛存在多种耐药菌。我们建议使用基于纳米技术的新型去污方法。

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