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首页> 外文期刊>Journal of medical bacteriology. >Contamination of Tap Water with Pseudomonas aeruginosa, Legionella pneumophila, and Escherichia coli in Guilan, Iran
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Contamination of Tap Water with Pseudomonas aeruginosa, Legionella pneumophila, and Escherichia coli in Guilan, Iran

机译：伊朗吉兰的铜绿假单胞菌，嗜肺军团菌和大肠杆菌对自来水的污染

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Background: River and underground waters are main sources of tap water in Guilan, Iran. Overland wastes move into rivers during periods of heavy or extended rain that is very common in the area.Pseudomonas aeruginosa, Legionella pneumophila, and Escherichia coli are main human pathogens with water source. This study is designed to determine the load of these bacteria in main water suppliesof the area.Methods: Samples were collected directly into sterile containers, concentrated by centrifuge,inoculated in enrichment medium and incubated for 3-4 days. DNA was extracted by using commercialkit. Several rounds of PCR was performed to search P. saeroginosa, integron I, Metallo-β-lactamases gene, L. pneumophila, mip gene, and E. coli.Results: About 92.0% of the samples showed bacterial contamination as revealed by PCR with primers of 16S rRNAgene, 9.5% of the samples had L. pneumophila, and 11,1% had Pseudomonas aeruginosa,but Escherichia coli was not detected. We found the mip gene in 66.6% of the samples with L.pneumophila. Metallo-β-lactamasesgene was found in 11.1% of all samples. We also found Integrin 1 in 28.5% of the samples with P. aeruginosa.Conclusion: This study indicates that in spite of chlorination, total bacterial contamination of pot waters in the area is high and contamination with L. pneumophila and P. aeroginosa is considerable. Itmight be related to the biofilm formation and the growth of water microflora. It seems that free residual chlorine is ineffective. We suggest a more effective decontamination procedure based on modern technology.
机译：背景：河流和地下水是伊朗桂兰的自来水的主要来源。在该地区非常普遍的大雨或长时间降雨期间，陆上废物进入河流。铜绿假单胞菌，肺炎军团菌和大肠杆菌是人类主要的水源病原体。方法：将样品直接收集到无菌容器中，通过离心浓缩，接种到富集培养基中，孵育3-4天。通过使用商用试剂盒提取DNA。进行了多轮PCR来搜寻铜绿假单胞菌，整合子I，金属β-内酰胺酶基因，肺炎支原体，mip基因和大肠杆菌。结果：大约92.0％的样品显示细菌污染，如用PCR检测到的。 16S rRNA基因的引物中，有9.5％的样本中有肺炎链球菌，而11.1％的样本中有铜绿假单胞菌，但未检出大肠杆菌。我们在66.6％的嗜肺乳杆菌样本中发现了mip基因。在所有样品中发现金属β-内酰胺酶基因的比例为11.1％。我们还发现在28.5％的铜绿假单胞菌样品中发现了整合素1。结论：尽管进行了氯化处理，但该地区的饮用水中细菌总数仍然很高，而肺炎嗜血杆菌和铜绿假单胞菌的污染仍然很大。 。这可能与生物膜的形成和水菌群的生长有关。游离的残留氯似乎无效。我们建议基于现代技术的更有效的去污程序。

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