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首页> 外文期刊>International Journal Bioautomation >Secondary Structural Models (16S rRNA) of Polyhydroxyalkanoates Producing Bacillus Species Isolated from Different Rhizospheric Soil: Phylogenetics and Chemical Analysis
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Secondary Structural Models (16S rRNA) of Polyhydroxyalkanoates Producing Bacillus Species Isolated from Different Rhizospheric Soil: Phylogenetics and Chemical Analysis

机译：从不同根际土壤中分离出产芽孢杆菌的多羟基链烷酸酯的二级结构模型（16S rRNA）：系统发生学和化学分析

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摘要

Polyhydroxyalkanoates (PHAs) producing bacterial isolates are gaining more importance over the world due to the synthesis of a biodegradable polymer which is extremely desirable to substitute synthetic plastics. PHAs are produced by various microorganisms under certain stress conditions. In this study, sixteen bacterial isolates characterized previously by partial 16S rRNA gene sequencing (NCBI Accession No. KF626466 to KF626481) were again stained by Nile red after three years of preservation in order to confirm their ability to accumulate PHAs. Also, phylogenetic analysis carried out in the present investigation evidenced that the bacterial species belonging to genus Bacillus are the dominant flora of the rhizospheric region, with a potentiality of biodegradable polymer (PHAs) production. Again, RNA secondary structure prediction hypothesized that there is no direct correlation between RNA folding pattern stability with a rate of PHAs production among the selected isolates of genus Bacillus .
机译：由于可生物降解的聚合物的合成，生产聚羟基链烷酸酯（PHA）的细菌分离物在世界范围内变得越来越重要，这是替代合成塑料极其需要的。 PHA由各种微生物在一定的压力条件下产生。在这项研究中，经过16年rSRNA基因部分测序（NCBI登录号KF626466至KF626481）表征的16个细菌分离株在保存三年后再次用尼罗红染色，以确认其积累PHAs的能力。而且，在本研究中进行的系统发育分析表明，属于芽孢杆菌属的细菌是根际区域的优势菌群，具有生物可降解聚合物（PHAs）产生的潜力。再次，RNA二级结构预测假设RNA折叠模式的稳定性与所选芽孢杆菌属菌株之间的PHA产生速率之间没有直接关系。

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